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Semin Liver Dis 2015; 35(01): 081-088
DOI: 10.1055/s-0034-1397352
DOI: 10.1055/s-0034-1397352
Adeno-Associated Virus-Mediated MicroRNA Delivery and Therapeutics
Further Information
Publication History
Publication Date:
29 January 2015 (online)
Abstract
MicroRNAs (miRNAs) are 20 to 24 nt long, single-stranded RNAs that repress gene expression. Dysregulation of miRNA expression is associated with many human diseases. Modulating the level of endogenous miRNA alters gene profiling and can achieve therapeutic benefits. Here the authors review currently used methods of altering miRNA activity in vivo. They focus on the delivery of miRNAs and miRNA inhibitors using recombinant adeno-associated virus (rAAV). In general, rAAV-mediated miRNA inhibition or overexpression provides a simple, efficient, and informative way to study miRNA function in mammals. This method also provides the opportunity to explore potential miRNA therapeutics for many diseases.
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