Int J Sports Med 2015; 36(14): 1163-1169
DOI: 10.1055/s-0035-1555851
Training & Testing
© Georg Thieme Verlag KG Stuttgart · New York

Skeletal Muscle Response to Endurance Training in IL-6−/− Mice

M. Wojewoda
1   Jagiellonian Centre for Experimental Therapeutics, Laboratory of Experimental Pharmacology of Endothelium, Jagiellonian University, Krakow, Poland
K. Kmiecik
1   Jagiellonian Centre for Experimental Therapeutics, Laboratory of Experimental Pharmacology of Endothelium, Jagiellonian University, Krakow, Poland
J. Majerczak
2   Department of Physiology and Biochemistry, University School of Physical Education, Krakow, Poland
R. Ventura-Clapier
3   University Paris-Sud, U-769 INSERM, Paris, France
D. Fortin
3   University Paris-Sud, U-769 INSERM, Paris, France
M. Onopiuk
4   Nencki Institute of Experimental Biology, Laboratory of Cellular Metabolism, Krakow, Poland
J. Rog
4   Nencki Institute of Experimental Biology, Laboratory of Cellular Metabolism, Krakow, Poland
K. Kaminski
5   Department of Cardiology, Medical University of Bialystok, Bialystok, Poland
S. Chlopicki
1   Jagiellonian Centre for Experimental Therapeutics, Laboratory of Experimental Pharmacology of Endothelium, Jagiellonian University, Krakow, Poland
6   Department of Experimental Pharmacology, Chair of Pharmacology, Jagiellonian University, Krakow, Poland
J. A. Zoladz
2   Department of Physiology and Biochemistry, University School of Physical Education, Krakow, Poland
› Author Affiliations
Further Information

Publication History

accepted after revision 08 June 2015

Publication Date:
28 October 2015 (online)


We examined effects of moderate-intensity endurance training on muscle COX/CS activities and V’O2max in control WT and IL-6−/− mice. Animals were exercised for 10 weeks on treadmill for 1 h, 5 days a week at velocity of 6 m·min−1 which was increased by 0.5 m·min−1 every 2 weeks up to 8 m·min−1 . Training triggered an increase of enzyme activities in soleus muscle of WT mice (COX: 480.3±8.9 U·g−1 in sedentary group vs. 773.3±62.6 U·g−1 in trained group, P<0.05 and CS: 374.0±6.0 U·g−1 in sedentary group vs. 534.2±20.5 U·g−1 in trained group, P<0.01, respectively) whereas no changes were observed in soleus of IL6−/− mice. Moreover, in mixed gastrocnemius muscle of trained IL-6−/− mice enzyme activities tended to be lower (COX: 410.7±48.4 U·g−1 for sedentary vs. 277.0±36.5 U·g−1 for trained group and CS: 343.8±24.6 U·g−1 for sedentary vs. 251.7±27.1 U·g−1 for trained group). No changes in V’O2max were observed in WT and IL-6−/− mice after training. Concluding, moderate-velocity endurance training-induced increase in COX and CS activities in muscles of WT mice only which suggests that IL-6 regulates training-induced skeletal muscle responses to exercise.

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