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Antioxidant capacity and phenolic profiling of Combretum collinum from Benin
27 September 2017 (online)
Traditional medicine is widely practised in Benin and plays an important role in addition to state healthcare . Combretum collinum is commonly used for the therapy of various ailments; especially the leaves are applied for the treatment of inflammatory conditions and wound healing .However, only preliminary phytochemical screenings have been carried out to identify bioactive compounds and an analysis of the phenolic profile of the leaves of Combretum collinum from Benin is warranted. Therefore, this research focuses on the characterisation of the phenolic profile and the antioxidant effects of an ethanolic leaf extract of Combretum collinum from Benin. A combined approach of chromatographic- and radical-scavenging activity screening of the ethanolic extract was applied for the identification of active compounds. A photometric method was used to quantify the antioxidant activity with the DPPH-radical-scavenging-assay. HPLC and LC-MS analysis of the major phenolic compounds identified the flavonoids myricetin-3-O-glucoside and myricetin-3-O-rhamnoside as the main phenolic compounds with antioxidant activity. The ethanolic leaf extract showed a phenolic content of 2.75 ± 0.34% and a radical-scavenging IC50 value of 13.94 ± 1.12 µg/ml in the DPPH-assay. The presence of the antioxidant compounds myricetin-3-O-glucoside and myricetin-3-O-rhamnoside supports the application of this plant for anti-inflammatory and wound healing purposes . Further studies of isolated fractions are needed to identify more main compounds and evaluate synergistic effects. In addition, pharmacodynamic and pharmacokinetic studies are required to characterise the underlying pharmacological mechanisms and evaluate the absorption of the compounds during topical and oral application.
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