Thromb Haemost 2000; 83(04): 589-591
DOI: 10.1055/s-0037-1613868
Commentary
Schattauer GmbH

The Thrombogram: Monitoring Thrombin Generation in Platelet Rich Plasma

Coenraad H. Hemker
2  Department of Biochemistry and Synapse BV, Cardiovascular Research Institute (CARIM) Maastricht, The Netherlands
,
Peter L. A. Giesen
2  Department of Biochemistry and Synapse BV, Cardiovascular Research Institute (CARIM) Maastricht, The Netherlands
,
Manoj Ramjee
1  Peptide Therapeutics Ltd., Cambridge, United Kingdom
,
Rob Wagenvoord
2  Department of Biochemistry and Synapse BV, Cardiovascular Research Institute (CARIM) Maastricht, The Netherlands
,
Suzette Béguin
2  Department of Biochemistry and Synapse BV, Cardiovascular Research Institute (CARIM) Maastricht, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 20 July 1999

Accepted after revision 23 November 1999

Publication Date:
08 December 2017 (online)

Summary

A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a clot or the presence of platelets and direct measurement in platelet rich plasma is possible.