Assessment of Primary Hemostasis by PFA-100® Analysis in a Tertiary Care Center

Thomas L. Ortel; Andra H. James; Elizabeth H. Thames; Karen D. Moore; Charles S. Greenberg

doi:10.1055/s-0037-1613974

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2000; 84(01): 93-97
DOI: 10.1055/s-0037-1613974

Commentary

Schattauer GmbH

Assessment of Primary Hemostasis by PFA-100® Analysis in a Tertiary Care Center

Authors

Thomas L. Ortel

¹From the Departments of Medicine, Pathology, and Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina, USA
Andra H. James

¹From the Departments of Medicine, Pathology, and Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina, USA
Elizabeth H. Thames

¹From the Departments of Medicine, Pathology, and Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina, USA
Karen D. Moore

¹From the Departments of Medicine, Pathology, and Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina, USA
Charles S. Greenberg

¹From the Departments of Medicine, Pathology, and Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina, USA

Abstract

Summary

We evaluated the utility of the PFA-100® platelet function analyzer in identifying disorders in platelet function and/or von Willebrand factor (vWF) in patients with various systemic disorders being followed at a tertiary care center. Closure times were determined with collagen/ ADP (CADP) and collagen/epinephrine (CEPI) cartridges for 305 patients, and abnormal results were further evaluated with platelet aggregometry and vWF analysis. Prolonged CADP and/or CEPI closure times were identified in 114 patients (37.3%), but most were isolated prolonged CEPI closure times predominantly due to aspirin therapy (79 patients). Prolonged CADP closure times were most frequently due to qualitative platelet defects and/or decreased vWF levels. Prolonged CADP closure times were encountered most frequently in patients with sickle cell disease and were associated with a decreased hematocrit. This study demonstrated that the PFA-100® analyzer can accurately assess vWF-dependent platelet function and detect other platelet defects under high shear stress in complex patient populations.

Keywords

Primary hemostasis - platelets - von Willebrand factor

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Referenzen

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