Free and Total Platelet Glycoprotein IIb/IIIa Measurement in Whole Blood by Quantitative Flow Cytometry during and after Infusion of c7E3 Fab in Patients Undergoing PTCA

Nathalie Hézard; Damien Metz; Pierre Nazeyrollas; Philippe Nguyen; Gérard Simon; Sylvie Daliphard; Chantal Droullé; Jacques Elaerts; Gérard Potron

doi:10.1055/s-0037-1614590

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1999; 81(06): 869-873
DOI: 10.1055/s-0037-1614590

Letters to the Editor

Schattauer GmbH

Free and Total Platelet Glycoprotein IIb/IIIa Measurement in Whole Blood by Quantitative Flow Cytometry during and after Infusion of c7E3 Fab in Patients Undergoing PTCA

Authors

Nathalie Hézard

¹From the Laboratoire Central d’Hématologie and, Reims, France
Damien Metz

²Service de Cardiologie, CHU Robert Debré, Reims, France
Pierre Nazeyrollas

²Service de Cardiologie, CHU Robert Debré, Reims, France
Philippe Nguyen

¹From the Laboratoire Central d’Hématologie and, Reims, France
Gérard Simon

¹From the Laboratoire Central d’Hématologie and, Reims, France
Sylvie Daliphard

¹From the Laboratoire Central d’Hématologie and, Reims, France
Chantal Droullé

¹From the Laboratoire Central d’Hématologie and, Reims, France
Jacques Elaerts

²Service de Cardiologie, CHU Robert Debré, Reims, France
Gérard Potron

¹From the Laboratoire Central d’Hématologie and, Reims, France

Abstract

Summary

A quantitative flow cytometry assay was used to evaluate the ex vivo kinetics of c7E3 Fab platelet effect in 16 patients undergoing PTCA treated with abciximab and compared with aggregometry assay. Immunolabeling of platelets was directly assessed on whole blood, using in parallel two monoclonal antibodies (Mabs) raised against GPIIIa, Mab1, the binding of which is inhibited by c7E3 Fab, and Mab2, the binding of which is not affected by c7E3 Fab. We found a severe and sustained inhibition of both GPIIb/IIIa receptors and platelet functions. The inter-individual variation in response to abciximab was low. A significant transient increase at H24 and H48 in the binding of Mab2 was found as an unexpected result, and confirmed in vitro. Results demonstrate that flow cytometry is a reliable method in agreement with aggregation. In addition, our results show that it is a standardized tool and a time-saving technique.

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Referenzen

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