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Thromb Haemost 1998; 79(03): 529-538
DOI: 10.1055/s-0037-1614939
Review Articles
Schattauer GmbH

Regulation of Monocyte Tissue Factor Activity by Allogeneic and Xenogeneic Endothelial Cells

Christoph W. Kopp
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Simon C. Robson
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Jonathan B. Siegel
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Josef Anrather
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Hans Winkler
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Shane Grey
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Elzbieta Kaczmarek
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Fritz H. Bach
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
,
Carolyn L. Geczy
1   From the Sandoz Center for Immunobiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; and The Heart Research Institute, Camperdown, Australia
› Author AffiliationsThis work was supported by grants from Sandoz Pharma, Basel, Switzerland and the National Health and Medical Research Council of Australia. C. W. K. holds a “Max Kade” fellowship from the Austrian Academy of Science. S. C. R. had support from the Medical Research Council Liver Center, University of Cape Town, South Africa.
Further Information

Publication History

Received 27 January 1997

Accepted after resubmission 27 October 1997

Publication Date:
07 December 2017 (online)

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Summary

The regulation of tissue factor (TF) activity by the cell associated tissue factor pathway inhibitor (TFPI) during monocyte (Mo) and endothelial cell (EC) interactions is not fully understood. This report describes co-ordinate induction of TF antigen (TF-Ag) and membrane-associated TFPI-Ag on human Mo following coculture with human aortic (HAEC) or porcine aortic EC (PAEC) or after stimulation with TNFα. We show that both allo- and xenogeneic EC induce human Mo-TF antigen in short-term culture. However, the TF activity of TNFα-primed Mo is suppressed when these cells are cocultured with HAEC [by 40.3 ± 6.3% (p <0.02)] or PAEC [by 50.5 ± 10.6% (p <0.001)]. Antibody (Ab) blocking studies confirm that TFPI is the principal anticoagulant associated with this suppression of TF-activity. Our data indicate that anti-TF activity originates, at least in part, from the activated human Mo in the coculture; additionally, specific generation of TFPI by Mo is observed under the xenogeneic culture conditions. As Mo associated TF, induced by allo- or xenogeneic EC interactions, is regulated by cell-associated TFPI, we propose that infiltrating Mo may modulate the thrombotic process at sites of vascular injury in association with both allo- and xenograft rejection.

Aspects of the work were presented at the American Society for Hematology meeting in Orlando, FL, December 1996.

 

  • References

  • 1 Geczy CL. Cellular mechanisms for the activation of blood coagulation. [Review]. Int Rev Cytol 1994; 152 (49) 49-108.
    PubMedGoogle Scholar
  • 2 Edgington TS, Mackman N, Brand K, Ruf W. The structural biology of expression and function of tissue factor. [Review]. Thromb Haemost 1991; 66 (01) 67-79.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 3 Edgington TS, Ruf W, Rehemtulla A, Mackman N. The molecular biology of initiation of coagulation by tissue factor. [Review]. Curr Stud Hemat Blood Trans 1991; 58: 15-21.
    PubMedGoogle Scholar
  • 4 Bach R, Gentry R, Nemerson Y. Factor VII binding to tissue factor in reconstituted phospholipid vesicles: induction of co-operativity by phosphatidylserine. Biochem 1986; 25: 4007-20.
    CrossrefPubMedGoogle Scholar
  • 5 Neuenschwander PF, Morrissey JH. Roles of the membrane-interactive regions of factor VIIa and tissue factor? The factor VIIa Gla domain is dispensable for binding to tissue factor but important for activation of factor X [published erratum appears in J Biol Chem 1994 Jun 17; 269(24): 16983]. J Biol Chem 1994; 269 (11) 8007-13.
    PubMedGoogle Scholar
  • 6 Neuenschwander PF, Fiore MM, Morrissey JH. Factor VII autoactivation proceeds via interaction of distinct protease-cofactor and zymogen-cofactor complexes. Implications of a two-dimensional enzyme kinetic mechanism. J Biol Chem 1993; 268 (29) 21489-92.
    PubMedGoogle Scholar
  • 7 Broze GJ, Girard TJ, Novotny WF. The lipoprotein-associated coagulation inhibitor. [Review]. Prog Hemost Thromb 1991; 10 (243) 243-68.
    PubMedGoogle Scholar
  • 8 Østerud B. The role of endothelial cells and subendothelial components in the initiation of blood coagulation. Haemostasis 1979; 8 (3-5): 324-31.
    PubMedGoogle Scholar
  • 9 Colucci M, Balconi G, Lorenzet R. et al. Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 1983; 71 (06) 1893-6.
    CrossrefPubMedGoogle Scholar
  • 10 Stern D, Nawroth P, Handley D, Kisiel W. An endothelial cell-dependent pathway of coagulation. Proc Natl Acad Sci 1985; 82 (08) 2523-7.
    CrossrefPubMedGoogle Scholar
  • 11 Nawroth PP, Stern DM. A pathway of coagulation on endothelial cells. J Cell Biol 1985; 28 (04) 253-64.
    CrossrefPubMedGoogle Scholar
  • 12 Lindahl AK, Sandset PM, Abildgaard U. The present status of tissue factor pathway inhibitor. [Review]. Blood Coag Fibrinol 1992; 3 (04) 439-49.
    CrossrefPubMedGoogle Scholar
  • 13 Pearson JD. Vessel wall interactions regulating thrombosis. [Review]. Br Med Bull 1994; 50 (04) 776-88.
    CrossrefPubMedGoogle Scholar
  • 14 Broze GJ. Tissue factor pathway inhibitor. [Review]. Thromb Haemost 1995; 74 (01) 90-3.
    PubMedGoogle Scholar
  • 15 Ameri A, Kuppuswamy MN, Basu S, Bajaj SP. Expression of tissue factor pathway inhibitor by cultured endothelial cells in response to inflammatory mediators. Blood 1992; 79: 3219-26.
    PubMedGoogle Scholar
  • 16 McGee MP, Foster S, Wang X. Simultaneous expression of tissue factor and tissue factor pathway inhibitor by human monocytes. A potential mechanism for localized control of blood coagulation. J Exp Med 1994; 179 (06) 1847-54.
    CrossrefPubMedGoogle Scholar
  • 17 Gray E, Thomas S, Mistry Y, Poole S. Inhibition of tissue factor and cytokine release. Haemostasis 1996; 26 (Suppl. 01) 92-5.
    PubMedGoogle Scholar
  • 18 van der Logt CP, Dirven RJ, Reitsma PH, Bertini RM. Expression of tissue factor and tissue factor pathway inhibitor in monocytes in response to bacterial lipopolysaccharide and phorbolester. Blood Coag Fibrinol 1994; 5 (02) 211-20.
    CrossrefPubMedGoogle Scholar
  • 19 Wharram BL, Fitting K, Kunkel SL, Remick DG, Merritt SE, Wiggins RC. Tissue factor expression in endothelial cell/monocyte cocultures stimulated by lipopolysaccharide and/or aggregated IgG. Mechanisms of cell:cell communication. J Immunol 1991; 146 (05) 1437-45.
    PubMedGoogle Scholar
  • 20 Lo SK, Cheung A, Zheng Q, Silverstein RL. Induction of tissue factor on monocytes by adhesion to endothelial cells. J Immunol 1995; 154 (09) 4768-77.
    PubMedGoogle Scholar
  • 21 Celi A, Pellegrini G, Lorenzet R. et al. P-selectin induces the expression of tissue factor on monocytes. Proc Natl Acad Sci 1994; 91 (19) 8767-71.
    CrossrefPubMedGoogle Scholar
  • 22 Millan MT, Geczy C, Stuhlmeier KM, Goodman DJ, Ferran CF, Bach FH. Human monocytes activate porcine endothelial cells, resulting in increased E-selectin, interleukin-8, monocyte chemotactic protein-1, and plasminogen activator inhibitor-type-1 expression. Transplantation 1997; 63: 421-9.
    CrossrefPubMedGoogle Scholar
  • 23 Kim PC, Cohen Z, Wong PY. et al. The effects of cyclosporine and cyclosporine metabolites in experimental small intestinal transplantation. Transplantation 1990; 49 (06) 1043-50.
    CrossrefPubMedGoogle Scholar
  • 24 Hancock WW, Sayegh MH, Sablinski T, Kut JP, Kupiec WJ, Milford EL. Blocking of mononuclear cell accumulation, cytokine production, and endothelial activation within rat cardiac allografts by CD4 monoclonal antibody therapy. Transplantation 1992; 53 (06) 1276-80.
    CrossrefPubMedGoogle Scholar
  • 25 Hancock WW, Gee D, De MP, Rickles FR, Ewan VA, Atkins RC. Immunohistological analysis of serial biopsies taken during human renal allograft rejection. Changing profile of infiltrating cells and activation of the coagulation system. Transplantation 1985; 39 (04) 430-8.
    CrossrefPubMedGoogle Scholar
  • 26 Bach FH, Robson SC, Winkler H. et al. Barriers to xenotransplantation [see comments]. [Review]. Nature Med 1995; 1 (09) 869-73.
    CrossrefPubMedGoogle Scholar
  • 27 Blakely ML, Van der Werf, Berndt M, Augustin P, Dalmasso AP, Bach FH, Hancock WW. Activation of intragraft endothelial and mononuclear cells during discordant xenograft rejection. Transplantation 1994; 58 (10) 1059-66.
    CrossrefPubMedGoogle Scholar
  • 28 Kopp CW, Siegel JB, Hancock WW, Anrather J, Winkler H, Geczy CL, Kaczmarek E, Bach FH, Robson SC. Effect of porcine tissue factor pathway inhibitor on activated human coagulation factors. Transplantation 1997; 63: 749-58.
    CrossrefPubMedGoogle Scholar
  • 29 Warren JB. An Introduction to Current Research. In: Warren JB. ed. The Endothelium. New York: Wiley-Liss, Inc.; 1990: 263.
    Google Scholar
  • 30 Hamamoto T, Yamamoto M, Nordfang O, Petersen JG, Foster DC, Kisiel W. Inhibitory properties of full-length and truncated recombinant tissue factor pathway inhibitor (TFPI). Evidence that the third Kunitz-type domain of TFPI is not essential for the inhibition of factor VIIa-tissue factor complexes on cell surfaces. J. Biol. Chem. 1993; 268 (12) 8704-10.
    PubMedGoogle Scholar
  • 31 Edwards RL, Rickles FR. The role of leukocytes in the activation of blood coagulation. [Review] Semin Hematol 1992; 29 (03) 202-12.
    PubMedGoogle Scholar
  • 32 Biemond BJ, Levi M, ten CH. et al. Complete inhibition of endotoxin-induced coagulation activation in chimpanzees with a monoclonal Fab fragment against factor VII/VIIa. Thromb Haemost 1995; 73 (02) 223-30.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 33 Tsuchida A, Salem H, Thomson N, Hancock WW. Tumor necrosis factor production during human renal allograft rejection is associated with depression of plasma protein C and free protein S levels and decreased intragraft thrombomodulin expression. J Exp Med 1992; 175 (01) 81-90.
    CrossrefPubMedGoogle Scholar
  • 34 Solberg S, Østerud B, Larsen T, Sorlie D. Lack of ability to synthesize tissue factor by endothelial cells in intact human saphenous veins. Blood Coag Fibrinol 1990; 1 (06) 595-600.
    PubMedGoogle Scholar
  • 35 Ryan J, Brett J, Tijburg P, Bach RR, Kisiel W, Stern D. Tumor necrosis factor-induced endothelial tissue factor is associated with subendothelial matrix vesicles but is not expressed on the apical surface. Blood 1992; 80 (04) 966-74.
    PubMedGoogle Scholar
  • 36 Wada H, Wakita Y, Shiku H. Tissue factor expression in endothelial cells in health and disease. [Review] Blood Coag Fibrinol 1995; 6: S26-31.
    CrossrefPubMedGoogle Scholar
  • 37 Cole EH, Cardella CJ, Schulman J, Levy GA. Monocyte procoagulant activity and plasminogen activator. Role in human renal allograft rejection. Transplantation 1985; 40 (04) 363-71.
    CrossrefPubMedGoogle Scholar
  • 38 Rothberger H, Barringer M, Meredith J. Increased tissue factor activity of monocytes/macrophages isolated from canine renal allografts. Blood 1984; 63 (03) 623-8.
    PubMedGoogle Scholar
  • 39 Kim P, Koh I, Cohen Z. Role of procoagulant activity (PCA) in allograft rejection. In: Levy GA, Cole EH. eds. Role of Procoagulant Activity in Health and Disease. Boca Raton: CRC Press; 1994: 177-90.
    Google Scholar
  • 40 Helin H, Edgington TS. Allogeneic induction of the human T cell-instructed monocyte procoagulant response is rapid and is elicited by HLA-DR. J Exp Med 1983; 158 (03) 962-75.
    CrossrefPubMedGoogle Scholar
  • 41 Carlos T, Kovach N, Schwartz B. et al. Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1. Blood 1991; 77 (10) 2266-71.
    PubMedGoogle Scholar
  • 42 Elices MJ, Osborn L, Takada Y. et al. VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA-4/fibronectin binding site. Cell 1990; 60 (04) 577-84.
    CrossrefPubMedGoogle Scholar
  • 43 Grey ST, Hancock WW. A physiologic anti-inflammatory pathway based on thrombomodulin expression and generation of activated protein C by human mononuclear phagocytes. J Immunol 1996; 156 (06) 2256-63.
    PubMedGoogle Scholar
  • 44 McGee MP, Teuschler H, Parthasarathy N, Wagner WD. Specific regulation of procoagulant activity on monocytes. Intrinsic pathway inhibition by chondroitin 4,6-disulfate. J Biol Chem 1995; 270 (44) 26109-15.
    CrossrefPubMedGoogle Scholar
  • 45 Ameri A, Kuppuswamy MN, Basu S, Bajaj SP. Expression of tissue factor pathway inhibitor by cultured endothelial cells in response to inflammatory mediators. Blood 1992; 79 (12) 3219-26.
    PubMedGoogle Scholar
  • 46 Rana SV, Reimers HJ, Pathikonda MS, Bajaj SP. Expression of tissue factor and factor VIIa/tissue factor inhibitor activity in endotoxin or phorbol ester stimulated U937 monocyte-like cells. Blood 1988; 71 (01) 259-62.
    PubMedGoogle Scholar
  • 47 Walsh JD, Geczy CL. Discordant expression of tissue factor antigen and procoagulant activity on human monocytes activated with LPS and low dose cycloheximide. Thromb Haemost 1991; 66 (05) 552-8.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 48 Bach R, Rifkin DB. Expression of tissue factor procoagulant activity: regulation by cytosolic calcium. Proc Natl Acad Sci 1990; 87 (18) 6995-9.
    CrossrefPubMedGoogle Scholar
  • 49 Østerud B, Bajaj MS, Bajaj SP. Sites of tissue factor pathway inhibitor (TFPI) and tissue factor expression under physiologic and pathologic conditions. On behalf of the Subcommittee on Tissue factor Pathway Inhibitor (TFPI) of the Scientific and Standardization Committee of the ISTH. [Review] Thromb Haemost 1995; 73 (05) 873-5.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 50 Sevinsky JR, Rao LV, Ruf W. Ligand-induced protease receptor translocation into caveolae: a mechanism for regulating cell surface proteolysis of the tissue factor-dependent coagulation pathway. J Cell Biol 1996; 133: 293-304.
    CrossrefPubMedGoogle Scholar
  • 51 Altieri DC. Coagulation assembly on leukocytes in transmembrane signaling and cell adhesion. [Review] Blood 1993; 81: 569-79.
    PubMedGoogle Scholar
  • 52 Duperray A, Languino LR, Plescia J, Mcdowall A, Hogg N, Craig AG, Berendt AR, Altieri DC. Molecular identification of a novel fibrinogen binding site on the first domain of ICAM-1 regulating leukocyte-endothelium bridging. J Biol Chem 1997; 272: 435-41.
    CrossrefPubMedGoogle Scholar
  • 53 Fan ST, Mackman N, Cui MZ, Edgington TS. Integrin regulation of an inflammatory effector gene. Direct induction of the tissue factor promoter by engagement of beta 1 or alpha 4 integrin chains. J Immunol 1995; 154: 3266-74.
    PubMedGoogle Scholar
  • 54 Napoleone E, Disanto A, Lorenzet R. Monocytes upregulate endothelial cell expression of tissue factor – a role for cell-cell contact and cross-talk. Blood. 1997; 89: 541-9.
    PubMedGoogle Scholar