Platelet Activation and Cytokine Production during Hypothermic Cardiopulmonary Bypass – A Possible Correlation?

P. Ferroni; G. Speziale; G. Ruvolo; A. Giovannelli; F. M. Pulcinelli; L. Lenti; P. Pignatelli; A. Criniti; E. Tonelli; B. Marino; P. P. Gazzaniga

doi:10.1055/s-0037-1615139

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 80(01): 58-64
DOI: 10.1055/s-0037-1615139

Rapid Communication

Schattauer GmbH

Platelet Activation and Cytokine Production during Hypothermic Cardiopulmonary Bypass – A Possible Correlation?

P. Ferroni

¹Department of Experimental Medicine and Pathology

,

G. Speziale

²Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy

,

G. Ruvolo

²Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy

,

A. Giovannelli

²Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy

,

F. M. Pulcinelli

¹Department of Experimental Medicine and Pathology

,

L. Lenti

¹Department of Experimental Medicine and Pathology

,

P. Pignatelli

¹Department of Experimental Medicine and Pathology

,

A. Criniti

²Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy

,

E. Tonelli

²Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy

,

B. Marino

²Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy

,

P. P. Gazzaniga

¹Department of Experimental Medicine and Pathology

› Author Affiliations

Abstract

Summary

Cardiopulmonary bypass (CPB) is associated with impaired platelet function and a systemic inflammatory response. The present study was designed to evaluate whether any correlation between platelet activation and inflammatory response during CPB exists. The results obtained from 8 patients undergoing hypothermic CPB for cardiac surgery showed the occurrence of a moderate degree of platelet activation during CPB, demonstrated by an increase of platelet CD62P expression in correlation with an increase of β-thromboglobulin levels, with a concomitant decrease of in vitro platelet response. Plasma IL-1β levels significantly increased during CPB, with a peak between 1 and 4 h after CPB. Similarly, IL-6 levels were elevated 30 min from CPB starting, peaked at 4 h, and remained elevated after 24 h. A direct correlation was found between plasma IL-1β and IL-6 levels. A significant correlation between plasma IL-1β and β-thromboglobulin levels was also found. In turn, plasma β-thromboglobulin levels correlated with CD62P expression on activated platelets. An inverse correlation was found between in vitro platelet aggregation and plasma IL-1β or IL-6 levels. From the present results it may be speculated that platelet activation during CPB may contribute, through the release of IL-1β, to activation of endothelial cells and subsequent release of other cytokines with chemotactic and pro-inflammatory properties, thus playing an important role in the inflammatory response associated with CPB.

Full Text

References

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