Thromb Haemost 1998; 80(02): 316-320
DOI: 10.1055/s-0037-1615194
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Reconstituted High Density Lipoprotein (rHDL) Modulates Platelet Activity In Vitro and Ex Vivo

Peter G. Lerch
1  From the ZLB Central Laboratory, Blood Transfusion Service SRC, Bern, Switzerland
,
Martin O. Spycher
1  From the ZLB Central Laboratory, Blood Transfusion Service SRC, Bern, Switzerland
,
Jan E. Doran
1  From the ZLB Central Laboratory, Blood Transfusion Service SRC, Bern, Switzerland
› Author Affiliations
Further Information

Publication History

Received 10 January 1997

Accepted after resubmission 22 April 1998

Publication Date:
08 December 2017 (online)

Summary

A reconstituted high density lipoprotein (rHDL) prepared for clinical use was tested for its influence on platelet activity modulated by various stimuli. In a first series of in vitro experiments, rHDL was added to blood in a concentration series, and platelet rich plasma (PRP) was isolated. Platelets were stimulated with arachidonic acid, collagen, epinephrine or ADP, and platelet aggregation was assessed. rHDL mediated a dose dependent inhibition of the platelet activity. With purified platelets rHDL inhibited the release reaction induced by collagen, but not by thrombin, as measured by CD62P (P-Selectin) expression on the plasma membrane. Ex vivo experiments were performed with PRP from volunteers, previously infused with 25 mg rHDL/kg body weight and 40 mg rHDL/kg body weight, respectively. Platelet activity in PRP was assessed before, and up to 30 h after the end of the rHDL infusion. A transient inhibition of the platelet aggregation induced by arachidonic acid and collagen was observed which was more pronounced in the group receiving 40 mg rHDL/kg body weight. In both groups of experiments, in vitro and ex vivo, the inhibition of the platelet activity was also dependent on the stimulus used.