Replacement of Dietary Saturated by Unsaturated Fatty Acids: Effects on Platelet Protein Kinase C Activity, Urinary Content of 2,3-dinor-TXB2 and In Vitro Platelet Aggregation in Healthy Man

Anu M. Turpeinen; Anne-Maria Pajari; Riitta Freese; Roswitha Sauer; Marja Mutanen

doi:10.1055/s-0037-1615437

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 80(04): 649-655
DOI: 10.1055/s-0037-1615437

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Replacement of Dietary Saturated by Unsaturated Fatty Acids: Effects on Platelet Protein Kinase C Activity, Urinary Content of 2,3-dinor-TXB₂ and In Vitro Platelet Aggregation in Healthy Man

Anu M. Turpeinen

¹From the Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, Helsinki, Finland

,

Anne-Maria Pajari

¹From the Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, Helsinki, Finland

,

Riitta Freese

¹From the Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, Helsinki, Finland

,

Roswitha Sauer

¹From the Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, Helsinki, Finland

,

Marja Mutanen

¹From the Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, Helsinki, Finland

› Author Affiliations

Abstract

Summary

The present study was conducted to compare the effects of oleic (OA) and linoleic acids (LA) on platelet function in healthy subjects. After a 4-week period on a diet rich in saturated fatty acids (SAFA), 38 volunteers (20 female, 18 male) had a high OA (18.0 en%) diet or a high LA diet (11.5 en%) for four weeks in a controlled manner. A control group of 13 subjects consumed their habitual diet throughout the study.

Replacing the SAFA diet by the OA or LA diet did not affect the membrane-associated activity of platelet protein kinase C (PKC). However, both diets tended to increase the cytosolic activity to a comparable extent. Both the OA and the LA diets increased urinary excretion of 2,3-dinor-TXB₂, as compared to the SAFA diet, whereas the urinary excretion of β-thromboglobulin remained unchanged. As compared to the initial SAFA diet, platelet aggregation to collagen increased after both diets, while ADP induced aggregation showed no diet-induced changes. The results indicate increased platelet activity after both oil diets with no differences between the OA and LA diet and confirm in vitro findings that cis-unsaturated fatty acids have a distinct effect on cytosolic PKC, in particular.

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References

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