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Tierarztl Prax Ausg G Grosstiere Nutztiere 2012; 40(01): 7-13
DOI: 10.1055/s-0038-1623092
Originalartikel
Schattauer GmbH

Wirksamkeit einer vaginalen Progesteronspange zur Verringerung embryonaler Verluste in der Frühträchtigkeit bei Milchkühen

Efficacy of progesterone inserts (CIDR) to reduce early embryonic losses in dairy cows
M. Pilz
1   Tierklinik für Fortpflanzung, Fachbereich Veterinärmedizin, Freie Universität Berlin
2   Tierärztliche Praxis G. Thiele, Baruth/Mark
,
C. Fischer-Tenhagen
1   Tierklinik für Fortpflanzung, Fachbereich Veterinärmedizin, Freie Universität Berlin
,
X. von Krueger
1   Tierklinik für Fortpflanzung, Fachbereich Veterinärmedizin, Freie Universität Berlin
,
G. Thiele
2   Tierärztliche Praxis G. Thiele, Baruth/Mark
,
W. Heuwieser
1   Tierklinik für Fortpflanzung, Fachbereich Veterinärmedizin, Freie Universität Berlin
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Eingegangen: 24. August 2011

Akzeptiert nach Revision: 14. November 2011

Publikationsdatum:
06. Januar 2018 (online)

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Zusammenfassung

Ziel der Studie war, den Einfluss einer intravaginalen Progesteronspange (CIDR®, Pfizer) auf embryonale Verluste zwischen Tag 27 und 39 post inseminationem (p. i.) zu testen. Geprüft wurden ferner die Hypothesen, dass die Progesteronspange die Serum-Progesteronkonzentration während der Anwendung beeinflusst und sich nach ihrer Entfernung keine negativen Auswirkungen auf das Fortbestehen der Trächtigkeit ergeben. Material und Methoden: Bei 74 Kühen eines Milchviehbetriebs in Brandenburg erfolgte eine zufällige Zuordnung zu zwei Gruppen. Diese Tiere waren nach Besamung als nichttragend diagnostiziert worden und wurden daraufhin hormonell in einem Ovsynch-Programm behandelt und terminiert besamt. Kühe der CIDR®-Gruppe (n = 36) erhielten an Tag 20 p. i. eine Progesteronspange, die an Tag 39 p. i. entfernt wurde. Kühe der Kontrollgruppe (n = 38) blieben unbehandelt. Blutproben zur Messung der Progesteronkonzentration mittels Immunoassay wurden an den Tagen 20, 27, 39 und 55 p. i. entnommen. Trächtigkeitsuntersuchungen fanden an Tag 27 p. i. mittels Ultraschall und an den Tagen 39 und 55 p. i. mittels rektaler Palpation statt. Ergebnisse: Die embryonalen Verluste zwischen Tag 27 und 39 p. i. betrugen insgesamt 37,1% (CIDR®-Gruppe: 35,3%; Kontrollgruppe: 38,9%; p = 0,83). Die Progesteronkonzentrationen unterschieden sich an Tag 20 p. i. zwischen den an Tag 39 p. i. trächtigen Tieren beider Gruppen nicht. An Tag 27 p. i. lag die Konzentration in der CIDR®-Gruppe mit 16,2 ± 9,9 ng/ ml höher als in der Kontrollgruppe mit 11,2 ± 3,4 ng/ml (p = 0,04). Nach Präparatentfernung zeigten Kühe der CIDR®-Gruppe an Tag 55 p. i. keine höheren Progesteronwerte als Kontrolltiere. Schlussfolgerung: Die Progesteronspange steigerte die Progesteron konzentration während der Anwendung und ihre Entfernung hatte keinen negativen Einfluss auf das Fortbestehen der Trächtigkeit . Klinische Relevanz: Die Supplementierung mit Progesteron an Tag 20 p. i. kann die embryonalen Verluste zwischen Tag 27 und 39 p. i. nicht verringern oder den Anteil tragender Tiere erhöhen.

Summary

The objective of this study was to test the efficacy of a progesterone releasing device (CIDR®, Pfizer, Germany) inserted on day (d) 20 post insemination (p. i.) to reduce embryonic losses between d 27 and 39 p. i. Furthermore, we hypothesized that CIDR® increases blood progesterone levels during the application period, but does not affect the maintenance of pregnancy after removal. Material and methods: The study was conducted on a commercial dairy farm, randomly allocating 74 Holstein Friesian cows to one of two groups. These cows were nonpregnant after previous artificial insemination and treated with an Ovsynch protocol. Group 1 (n = 36) received a CIDR® on d 20 p. i. (CIDR® group) while group 2 (n = 38) remained untreated (control group). CIDR® was removed on d 39 p. i. Blood samples were drawn from all cows on d 20, 27, 39 and 55 p. i. for analysis of progesterone (P4) concentrations by immunoassay (ADVIA Centaur®, Siemens, Germany). Pregnancy diagnosis was conducted on d 27 p. i. with ultrasonography, and on d 39 and 55 p. i. by transrectal palpation. Results: The overall prevalence rate of early embryonic losses between d 27 and 39 p. i. was 37.1% (CIDR® group 35.3%, control group 38.9%; p = 0.83). On d 39 p. i. 30.5% of all cows were pregnant and the percentage of pregnant cows did not differ between the study groups (p = 0.83). Progesterone levels on d 20 p. i. did not differ among cows which were pregnant on d 39 p. i. (p = 0.57). On d 27 p. i. progesterone levels in pregnant cows were higher in the CIDR® group (16.2 ± 9.9 ng/ml) compared to the control group (11.2 ± 3.4 ng/ml; p = 0.04). Progesterone concentrations were lower on d 39 p. i., but still differed between study groups (p = 0.05). After removal of CIDR®, blood progesterone levels did not differ between pregnant cows of both study groups on d 55 p. i. (p = 0.36). Conclusion: The application of a progesterone releasing device led to increased blood progesterone levels during the application period, but did not affect maintenance of pregnancy after its removal. Clinical relevance: Supplementation with progesterone at d 20 p. i. does not decrease early embryonic losses between d 27 and 39 p. i. or increase the number of cows pregnant on d 39 p. i.

Schlüsselwörter

Rind - Trächtigkeit - embryonale Verluste - Progesteron - CIDR® - Fertilität

Key words

Cattle - pregnancy - pregnancy loss - progesterone - CIDR® - fertility
 

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