Determination of DNA damage in vitro

Summary

Ionising irradiation acts primarily via induction of DNA damage, among which doublestrand breaks are the most important lesions. These lesions may lead to lethal chromosome aberrations, which are the main reason for cell inactivation. Double-strand breaks can be repaired by several different mechanisms. The regulation of these mechanisms appears be fairly different for normal and tumour cells. Among different cell lines capacity of doublestrand break repair varies by only few percents and is known to be determined mostly by genetic factors. Knowledge about doublestrand break repair mechanisms and their regulation is important for the optimal application of ionising irradiation in medicine.

Keywords

DNA double-strand breaks - cell inactivation - chromosome aberration - apoptosis - G1 arrest - DNA repair - non-homologous endjoining - homologous recombination

• Literatur

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