Gene expression analysis to examine the adhesion of the human neuroblastoma cell line SH-SY5Y growing on gold surfaces coated with L1CAM

Introduction:

Despite the technological proceeding of cochlear implant development the nerv-electrode interface still need to be optimised. Large distances between the synapses of spiral ganglion neurons (SGN) and the electrode contacts may result in simultaneous stimulation of the SGN bunches and, thus, diminish speech perception. Aim of the project is the induction of neurite outgrowth and subsequent adhesion on electrode surface coated with neuronal adhesion molecules of the IgCAM superfamily. So far, L1CAM represents a well characterized member of this IgCAM super family and was recently shown to be expressed in the inner ear and also in the human neuroblastoma cell line SH-SY5Y.

Methods:

A gene expression analysis by using the cDNA microarray technique was performed to identify adhesion molecules, integrins and extracellular matrix proteins in the SH-SY5Y cells growing on gold surfaces coated with 50 µg/ml L1CAM. Gold plates without any coatings were used as reference. Additionally, the cell culture medium was supplied with retinoic acid to induce neurite outgrowth in SH-SY5Y-cells. Following cultivation for 5 d total RNA was extracted for cDNA microarray analysis.

Results:

All in all, it could not be stated, that L1CAM coating favor adhesion and neurite outgrowth of the SH-SY5Y cells. The administration of RA to the culture medium induced the expression numerous adhesion related proteins independently of the surface structure.

Conclusion:

It may be assumed, that the appropriate transcript variants, i.e. for L1CAM, were not spotted onto the chip. Otherwise, uncoated gold surfaces may present optimal conditions for cell attachment, that cannot be surpassed by L1CAM.

Publication History

Publication Date:
18 April 2018 (online)