CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S338-S339
DOI: 10.1055/s-0038-1640892
Abstracts
Rhinologie: Rhinology

Nasal microbiome of patients with granulomatosis with polyangiitis

I Rolfs
1   UKSH Kiel/Klinik für HNO, Kopf-und Halschirurgie, Kiel
,
P Lamprecht
2   Universitätsklinikum Schleswig-Holstein, Lübeck/Klinik für Rheumatologie und k, Lübeck
,
N Fischer
3   Universitätsklinikum Hamburg Eppendorf/Institut für Medizinische Mikrobiologie, Hamburg
,
M Laudien
4   Universitätsklinikum Schleswig-Holstein, Kiel/Klinik für HNO, Kopf- und Halsch, Kiel
› Author Affiliations
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Introduction:

Inflammatory diseases are linked to a dysbiosis of the local microbiome. The objective of this study was to determine whether there is a specific composition of the nasal microbiome of patients with granulomatosis with polyangiitis (GPA).

Methods:

63 nasal swabs of 26 patients with GPA, 21 patients with rheumatoid arthritis (RA) and 27 control patients (CPs) were taken in the Department of ENT, head and neck surgery of the Christian- Albrechts- University of Kiel and the Department of Rheumatology of the University of Lübeck. The swabs were processed for standard microbial analysis using 16S rRNA amplicon sequencing to survey the composition of the nasal microbiome. Due to frequent detection of Staphylococcus aureus (S. aureus) in recent findings a specific diagnostic polymerase chain reaction (PCR) for these organisms was initiated as well.

Results:

The 16S rRNA analysis did not show any statistically significant difference in both the alpha- and beta-diversity between all patient groups. The PCR detected a higher rate of S. aureus in GPA patients (15/19) compared to CPs (4/23, p < 0.0005) and RA patients (9/25; p = 0.0046).

Conclusions:

The PCR analysis suggest a specific local microbiome with increased existence of S. aureus in patients with GPA compared to the control groups.



Publication History

Publication Date:
18 April 2018 (online)

© 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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