CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S375
DOI: 10.1055/s-0038-1641018
Abstracts
Speicheldrüsen/Schilddrüsen: Salivary Glands/Thyroid Gland

Raman spectroscopy (RS) for entity and dignity determination of salivary gland tumors

T Meyer
1   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, Würzburg
,
M Steinke
2   Lehrstuhl für Tissue Engineering & Regenerative Medizin der Universität Würzburg, Würzburg
,
E Hartmann
3   Pathologisches Institut der Universität Würzburg, Würzburg
,
R Hagen
4   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und äs, Würzburg
,
A Scherzad
4   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und äs, Würzburg
,
S Hackenberg
4   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und äs, Würzburg
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Introduction:

The pre- and intraoperative determination of the entity of salivary gland tumors (ST) is challenging. Entity and dignity classification only by histological and morphological criteria is not reliable.

Raman spectra are dependent on the molecular composition of the analyzed tissue.

The aim of the study was to analyze the impact of ST. In addition, a review for the application of RS in head and neck topics will be given.

Material and methods:

10 m thick, deparaffined slices of adenoid cystic carcinomas (n = 5) und pleomorphic adenomas (n = 5) were investigated by RS in a pilot study. The measurement was performed by the BioRam System of CellTool after stimulation with a 785nm diode laser in the tumor cells. „The Unscrambler“ X 10.3 Software was used for multivariate data analysis.

Results:

The averaging spectrum showed significant differences dependent of the tumor entity. In the principal component analysis the main differences were in the 2. and 3. principal component. The main differences were observed by 1665nm, 1240nm und 1060nm. In a „leave-one-out“-model a sensitivity of 79% and a specificity of 69% were reached.

Discussion:

Recent studies on RS in the head and neck area mainly focused on the discrimination of malignant mucosa from healthy tissue. Our data showed that the biological information assessed by RS could also be useful to determine the entity of ST. The accuracy in comparison to a histological investigation of aspiration cytology seems to be comparable.



Publication History

Publication Date:
18 April 2018 (online)

© 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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