Download PDF
Thromb Haemost 1988; 59(02): 231-235
DOI: 10.1055/s-0038-1642760
Original Articles
Schattauer GmbH Stuttgart

Monocytes of Patients Congenitally Deficient in Plasma Factor XIII Lack Factor XIII Subunit A Antigen and Transglutaminase Activity

László Muszbek
1   The Department of Clinical Chemistry, University School of Medicine, Debrecen, Hungary
,
Roza Ádány
1   The Department of Clinical Chemistry, University School of Medicine, Debrecen, Hungary
,
Mária Kávai
2   IIIrd Department of Medicine, University School of Medicine, Debrecen, Hungary
,
Zoltán Boda
3   IInd Department of Medicine, University School of Medicine, Debrecen, Hungary
,
Stanislaw Lopaciuk
4   Institute of Haematology, Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Received 12 October 1987

Accepted after revision 27 November 1987

Publication Date:
21 May 2018 (online)

  PDF Download  Permissions and Reprints

Summary

Monocytes isolated from patients with severe deficiency in plasma Factor XIII of blood coagulation (FXIII) were tested for FXIII antigen and transglutaminase activity. By immunoperoxi- dase method the patients’ monocytes, in contrast to normal controls, showed no reaction with a monospecific antibody against FXIII subunit ª.This result was confirmed by immuno- blotting technique, as well. In addition, tissue macrophages tested in one of the patients were also exempt of FXIII subunit ª antigen. The transglutaminase activity in FXIII deficient monocytes was below the limit of the detection of the dansylcadaverine incorporation assay. The results suggest that FXIII subunitªof monocytes/macrophages and its plasma and platelet counterparts are closely related or identical proteins and demonstrate that the transglutaminase activity in monocytes is of FXIII origin and tissue transglutaminase is present, if at all, only in insignificant amount.

Keywords

Factor XIII - Monocytes - Transglutaminase
 

  • References

  • 1 Lorand L, Losowsky MS, Miloszewski KJ M. Human factor XIII: fibrin-stabilizing factor. Progr Haemostas Thrombos 1980; 5: 245-290
    PubMedGoogle Scholar
  • 2 McDonagh J. Structure and function of Factor XIII. In: Haemostasis and Thrombosis. Colman RW, Hirsh J, Marder VJ, Salzman EW. (eds) pp 164-173 Lippincott; Philadelphia: 1982
    Google Scholar
  • 3 Muszbek L, Laki K. Interaction of thrombin with proteins other than fibrinogen (thrombin susceptible bonds). Activation of FXIII. In: The Thrombin Machovich R. (ed) pp 321-342 CRC Press; Boca Raton: 1984
    Google Scholar
  • 4 Muszbek L, Adany R, Szegedi G, Polgar J, Kavai M. FXIII of blood coagulation in human monocytes. Thrombos Res 1985; 37: 401-410
    CrossrefPubMedGoogle Scholar
  • 5 Adany R, Belkin A, Vasilevskaya T, Muszbek L. Identification of blood coagulation factor XIII in human peritoneal macrophages. Eur J Cell Biol 1985; 38: 171-173
    PubMedGoogle Scholar
  • 6 Henriksson P, Becker S, Lynch G, McDonagh J. Identification of intracellular Factor XIII in human monocytes and macrophages. J Clin Invest 1985; 76: 528-534
    CrossrefPubMedGoogle Scholar
  • 7 Adany R, Nemes Z, Thomazy V, Muszbek L. Identification of Factor XIII in macrophages invading lymph nodes with Hodgkin’s disease. In: Haemostasis and Cancer. Muszbek L. (ed) pp 125-134 CRC Press; Boca Raton: 1987
    Google Scholar
  • 8 Adany R, Nemes Z, Muszbek L. Characterization of Factor XIII containing macrophages in lymph nodes with Hodgkin’s disease. Brit J Cancer 1987; 55: 421-426
    CrossrefPubMedGoogle Scholar
  • 9 Adany R, Kappelmayer J, Muszbek L. Expression of factor XIII subunit A in different types of human macrophages. In: The Biology of Phagocytes in Health and Disease Mauri C, Rizzo SC, Ricevuti G. (eds) Adv Biosci 1987; 66: 323-333
    PubMedGoogle Scholar
  • 10 Murtaugh MP, Arend WP, Davies PJ A. Induction of tissue transglutaminase in human peripheral blood monocytes. J Exp Med 1984; 159: 114-125
    CrossrefPubMedGoogle Scholar
  • 11 Freundlich B, Avdalovic N. Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes. J Immunol Methods 1983; 62: 31-37
    CrossrefPubMedGoogle Scholar
  • 12 Pawlowski NA, Kaplan G, Hamill AL, Cohn ZA, Scott WA. Arachidonic acid metabolism by human monocytes. Studies with platelet-depleted cultures. J Exp Med 1983; 158: 393-412
    CrossrefPubMedGoogle Scholar
  • 13 Yam LT, Li CY, Crosby WH. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol 1971; 55: 283-290
    CrossrefPubMedGoogle Scholar
  • 14 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 277: 680-682
    PubMedGoogle Scholar
  • 15 Lorand L, Urayama T, deKiewiet JW C, Nossel HL. Diagnostic and genetic studies on fibrin-stabilizing factor with a new assay based on amine incorporation. J Clin Invest 1969; 48: 1054-1064
    CrossrefPubMedGoogle Scholar
  • 16 Janossy G, Bofill M, Poulter LW, Rawlings E, Burford GD, Navarete C, Ziegler A, Kelemen E. Separate ontogeny of two macrophage-like accessory cell populations in the human fetus. J Immunol 1986; 136: 4354-4361
    PubMedGoogle Scholar
  • 17 McDonagh J, McDonagh RP, Delage JM W, Wagner RH. Factor XIII in human plasma and platelets. J Clin Invest 1969; 48: 940-946
    CrossrefPubMedGoogle Scholar
  • 18 Folk JE. Transglutaminases. Ann Rev Biochem 1980; 49: 517-531
    CrossrefPubMedGoogle Scholar
  • 19 Schroff G, Neumann C, Sorg C. Transglutaminase as a marker of subsets of murine macrophages. Eur J Immunol 1981; 11: 637-642
    CrossrefPubMedGoogle Scholar
  • 20 Adany R, Kiss A, Muszbek L. Factor XIII: a marker of mono- and megakariocytopoesis. Br J Haematol 1987; 67: 167-72
    CrossrefPubMedGoogle Scholar
  • 21 Adany R, Glukhova MA, Kabakov AY, Muszbek L. Characterization of human connective tissue cells containing factor XIII subunit a. J Clin Pathol 1987; 41: 49-56
    PubMedGoogle Scholar
  • 22 Kradin RL, Lynch GW, Kurnick JT, Erikson M, Colvin RB, McDonagh J. Factor XIII is synthetized and expressed on the surface of U937 cells and alveolar macrophages. Blood 1987; 69: 778-785
    PubMedGoogle Scholar
  • 23 Weisberg LJ, Shin DT, Concling PR, Shuman MA. Identification of normal human peripheral blood monocytes and liver as sites of synthesis of coagulation Factor XIII a-chainZ. Blood 1987; 70: 579-582
    PubMedGoogle Scholar
  • 24 Shelley WB, Juhlin L. Induction of fibrin thrombi by monocytes. Nature 1977; 270: 343-344
    CrossrefPubMedGoogle Scholar
  • 25 Lorenzet R, Peri G, Locati D, Allavena P, Colucci M, Semeraro N, Mantovani A, Donati MB. Generation of procoagulant activity by mononuclear phagocytes: a possible mechanism contributing to blood clotting activation within malignant tissues. Blood 1981; 62: 271-273
    PubMedGoogle Scholar
  • 26 Rickies ER, Edwards RL. Activation of blood coagulation in cancer: Trousseau’s syndrome revisited. Blood 1983; 62: 14-31
    PubMedGoogle Scholar
  • 27 Dvorak HF, Senger RD, Dvorak AM. Fibrin as a component of the tumor stroma: origins and biological significance. Cancer Metast Rev 1983; 2: 41-73
    CrossrefPubMedGoogle Scholar
  • 28 Osterud B, Lindahl U, Seljelid R. Macrophages produce blood coagulation factors. FEBS Letters 1980; 120: 41-43
    CrossrefPubMedGoogle Scholar
  • 29 Lindahl U, Kolset SO, Bogwald J, Osterud B, Seljelid R. Studies with a luminogenic substrate on blood coagulation factor X/Xa produced by mouse peritoneal macrophages. Biochem J 1982; 206: 231-237
    CrossrefPubMedGoogle Scholar
  • 30 Chapman HA, Allen LC, Stone OL. Human alveolar macrophages synthesize factor VII in vitro. J Clin Invest 1985; 75: 2030-2037
    CrossrefPubMedGoogle Scholar
  • 31 Van Dam-Mieras MC E, Muller DA, Van Deijk WA, Hemker HC. Clotting factors secreted by monocytes and macrophages: analytical considerations. Thrombos Res 1985; 37: 9-19
    CrossrefPubMedGoogle Scholar
  • 32 Duckert F. Documentation of the plasma factor XIII deficiency in man. Ann NY Acad Sci 1972; 202: 190-199
    CrossrefPubMedGoogle Scholar
  • 33 Mosher DF, Schad PE, Vann JM. Cross-linking of collagen and fibronectin by Factor XHIa. J Biol Chem 1980; 255: 1181-1188
    PubMedGoogle Scholar
  • 34 Leibovich SJ, Ross R. The role of the macrophage in wound repair. Am J Pathol 1975; 78: 71-100
    PubMedGoogle Scholar
  • 35 Muszbek L, Adany R, Lopaciuk S, Kavai M. Possible role of Factor XIII subunit a in phagocytosis by monocytes. Haematol Rev Commun Suppl 1986; 1: 120a Abstr
    PubMedGoogle Scholar