Thromb Haemost 1990; 63(03): 454-458
DOI: 10.1055/s-0038-1645065
Original Article
Schattauer GmbH Stuttgart

The Simultaneous Acute Release of Tissue-Type Plasminogen Activator and von Willebrand Factor in the Perfused Rat Hindleg Region

N Tranquille
The Gaubius Institute TNO, Leiden, The Netherlands
J J Emeis
The Gaubius Institute TNO, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 27 September 1989

Accepted after revision 17 January 1990

Publication Date:
30 June 2018 (online)


In perfused rat hindlegs, platelet-activating factor and bradyki-nin induced the acute release of both tissue-type plasminogen activator (t-PA) and von Willebrand Factor (vWF). The time course of release was similar for both proteins, and the amounts of t-PA and vWF released under various conditions were closely correlated. Release of both t-PA and vWF required extracellular calcium, and could be induced by the calcium ionophore A-23187. Protein synthesis was not required for release to occur.

Phorbol myristate acetate also induced release of t-PA and vWF, though with a different time course; DDAVP was inactive.

The results suggest that the release of t-PA, and that of vWF, are closely linked at the cellular level.