Download PDF
Thromb Haemost 1987; 58(03): 911-914
DOI: 10.1055/s-0038-1646014
Original Article
Schattauer GmbH Stuttgart

Platelet Density-Dependent Partition of Platelet- von Willebrand Factor Between Alpha Granule and Non-Alpha Granule Pools

R I Parker
The Hematology Service, Clinical Pathology Department, Clinical Center National Institutes of Health, Bethesda, MD, USA
,
Brenda C Shafer
The Hematology Service, Clinical Pathology Department, Clinical Center National Institutes of Health, Bethesda, MD, USA
,
H R Gralnick
The Hematology Service, Clinical Pathology Department, Clinical Center National Institutes of Health, Bethesda, MD, USA
› Author Affiliations
Further Information

Publication History

Received 27 January 1987

Accepted after revision 11 June 1987

Publication Date:
28 June 2018 (online)

  PDF Download  Permissions and Reprints

Summary

In this study, we demonstrate that platelets contain a small hut significant amount of platelet-von Willebrand factor(vWf) not associated with α-granules. When platelets free of plasma proteins are exposed to micromolar concentrations ofdigitonin, plasma membrane permeabilization occurs without disruption of platelet granules. Employing this technique, wehave found that upon exposure of a total platelet population to 8 μM digitonin, 5% of total platelet-vWf is released into the supernatant; this occurs without release of (β-TG from α-granules. When platelets of discrete buoyant density profiles are tested, this extragranular platelet-vWf increased with decreasing platelet density. These findings suggest that a redistribution of platelet-vWf from α-granule to non-granule sites occurs coincident with a decrease inplatelet buoyant density.

Keywords

Platelet-vWf - Platelet density - Alpha granules
 

  • References

  • 1 Slot JW, Bouma BN, Montgomery R, Zimmerman TS. Platelet Factor VUI-related Antigen: Immunofluoresccnt Localization. Thromb Res 1978; 13: 871-881
    CrossrefPubMedGoogle Scholar
  • 2 Zucker MB, Brockman MJ, Kaplan KL. Factor VIII-related Antigen in Platelets. Localization and Release by Thrombin and Collagen. J Lab Clin Med 1979; 94: 675-682
    PubMedGoogle Scholar
  • 3 Wencel-Drake JD, Painter RG, Zimmerman TS, Ginsberg MH. Ultrastructural Localization of Human Platelet Thrombospondin, Fibrinogen, Fibronectin and von Willebrand Factor in Frozen Thin Section. Blood 1985; 65: 929-938
    CrossrefPubMedGoogle Scholar
  • 4 Howard MA, Montgomery DC, Hardisty RM. Factor Vlll-related Antigen in Platelets. Thromb Res 1974; 4: 617-624
    CrossrefPubMedGoogle Scholar
  • 5 Corash L, Mok Y. Platelet Heterogeneity: Density Distribution of Alpha Granule and Lysosomal Constituents in Native and Thrombin Stimulated Platelet Density Cohorts. Blood 1984; 64 (05) Suppl 1): 245 a, Abstr 890
    PubMedGoogle Scholar
  • 6 Corash L, Costa JL, Shafer B, Donlon JA, Murphy D. Heterogeneity of Human Whole Blood Platelet Subpopulations III. Density-Dependent Differences in Subcellular Constituents. Blood 1984; 64: 185-193
    PubMedGoogle Scholar
  • 7 Zuurendonck PF, Tager FM. Rapid Separation of Particulate Components and Soluble Cytoplasm of Isolated Rat-liver Cells. Biochim Biophys Acta 1974; 333: 393-399
    CrossrefPubMedGoogle Scholar
  • 8 Akkerman J WN, Ebberink R HM, Lips J PM, Christiaens G CM L. Rapid Separation of Cytosol and Particle Fraction of Human Platelets by Digitonin-induced Cell Damage. Br. J. Haem. 1980; 44: 291-300
    CrossrefPubMedGoogle Scholar
  • 9 Parker RI, Rick ME, Gralnick HR. A Method to Minimize Platelet Activation during Platelet Isolation. Thromb Res 1984; 36: 265-270
    CrossrefPubMedGoogle Scholar
  • 10 Corash L, Tan H, Gralnick HR. Heterogeneity of Human Whole Blood Platelet Subpopulations. I. Relationship Between Buoyant Density, Cell Volume, and Ultrastructure. Blood 1977; 49: 71-87
    PubMedGoogle Scholar
  • 11 Parker RI, Rick ME, Gralnick HR. The Effect of Calcium on the Availability of Platelet von Willebrand Factor. J Lab Clin Med 1985; 106: 336-342
    PubMedGoogle Scholar
  • 12 Hoyer LW. Immunologic Studies of Antihemophilic Factor (AHF, Factor VIII). IV. Radioimmunoassay ot AHF Antigen. J Lab Clin Med 1972; 80: 922-933
    PubMedGoogle Scholar
  • 13 Hoyer LW, Shainoff JR. Factor-Vlll-related Protein Circulates in Normal Human Plasma as High Molecular Weight Multimers. Blood 1980; 55: 1056-1059
    PubMedGoogle Scholar
  • 14 Parker RI, Rick ME, Gralnick HR. Absence of the Largest von Willebrand Factor Multimers in the Platelets of a Patient with Lactoferrin Deficiency and a Bleeding Tendency. Clin Res 1984; 32 (02) 318 A
    PubMedGoogle Scholar
  • 15 Gralnick HR, Rick MR, McKeown LP, Williams SB, Parker RI, Maisonneuve P, Jeanneau C, Sultan Y. Platelet, von Willebrand Factor: An Important Determinant of the Bleeding in Type I von Willebrand’s Disease. Blood 1986; 68: 58-61
    PubMedGoogle Scholar
  • 16 Jeanneau C, Sultan Y. Immunocytochemical Localization of Factor VUI/von Willebrand Factor Antigen in Human Platelets. Biol Cell 1983; 49: 237-242
    PubMedGoogle Scholar
  • 17 Wencel-Drake JD, Plow EF, Zimmerman TS, Painter RG, Ginsberg MH. Immunolocalization of Adhesive Glycoproteins in Resting and Thrombin-Stimulated Platelets. Am J Pathol 1984; 115: 156-164
    PubMedGoogle Scholar
  • 18 Stenberg PE, Shuman MA, Levine SP, Bainton DF. Redistribution of Alpha-Granules and their Contents in Thrombin-Stimulated Platelets. J Cell Biol 1984; 98: 748-760
    CrossrefPubMedGoogle Scholar
  • 19 Parker RI, Gralnick HR. Identification of Platelet Glycoprotein IIb/IIIa as the Major Binding Site for Released Platelet von Willebrand Factor. Blood 1986; 68: 732-736
    PubMedGoogle Scholar