Redistribution of Platelet Glycoproteins Induced by Allo- and Autoantibodies

S Santoso; V Kiefel; C Mueller-Eckhardt

doi:10.1055/s-0038-1646022

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1987; 58(03): 866-871
DOI: 10.1055/s-0038-1646022

Original Article

Schattauer GmbH Stuttgart

Redistribution of Platelet Glycoproteins Induced by Allo- and Autoantibodies

Authors

S Santoso

¹The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
V Kiefel

¹The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG
C Mueller-Eckhardt

¹The Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Gießen, FRG

Abstract

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Summary

The redistribution of platelet glycoproteins (GP) Ib, IIb and IIIa in response to stimulation with human platelet specific alloantibodies, autoantibodies and a quinidine-dependent antibody was investigated using immunofluorescence and a quantitative radioimmunoassay. The platelet GPs carrying the corresponding epitopes were determined using immunoblot technique or radioimmunoprecipitation. In accordance with previous results obtained with murine monoclonal platelet specific antibodies, redistribution upon stimulation with human platelet reactive antibodies was observed only due to reactions with epitopes on GP IIb, IIIa, respectively, but not on GP Ib. We therefore believe that membrane redistribution of human platelets induced by various antibodies is a function of the GP recognized by those antibodies rather than the source of the platelet reactive antibody.

Keywords

Platelet glycoprotein - Platelet specific antibodies - Redistribution - Endocytosis

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References

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