Selective Staining of Platelet Glycoproteins Using Two-Dimensional O’Farrell Gel Electrophoresis and Avidin-Biotin-Conjugated Lectins

Beate Kehrel; Renate Kokott; Leopold Balleisen; Werner Stenzinger; Kenneth J Clemetson; Jürgen van de Loo

doi:10.1055/s-0038-1646036

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1987; 58(04): 960-963
DOI: 10.1055/s-0038-1646036

Original Article

Schattauer GmbH Stuttgart

Selective Staining of Platelet Glycoproteins Using Two-Dimensional O’Farrell Gel Electrophoresis and Avidin-Biotin-Conjugated Lectins

Authors

Beate Kehrel

¹The Institute for Arteriosclerosis Research at the University of Münster, University of Berne, Berne, Switzerland
Renate Kokott

¹The Institute for Arteriosclerosis Research at the University of Münster, University of Berne, Berne, Switzerland
Leopold Balleisen

²The Department of Internal Medicine, University of Münster, Münster, Federal Republic of Germany
Werner Stenzinger

²The Department of Internal Medicine, University of Münster, Münster, Federal Republic of Germany
Kenneth J Clemetson

³The Theodor Kocher Institute, University of Berne, Berne, Switzerland
Jürgen van de Loo

²The Department of Internal Medicine, University of Münster, Münster, Federal Republic of Germany

Abstract

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Summary

A time-saving and sensitive high resolution method lor the analysis and identification of platelet glycoproteins using non-radioactive compounds has been developed. Platelet proteins (50 μg) of normal subjects were separated by isoelectric focusing and SDS-PAGE. Proteins were either stained with silver or electroblotted onto nitrocellulose. Nitrocellulose blots were treated with the following biotinylated lectins : Abrus precatorius lectin, concanavalin A, Lens culinaris lectin, or wheat germ agglutinin. Staining was achieved by avidin-biotin-coupled peroxidase using 4-chloro-l-naphthol as the substrate. A rapid overview of platelet glycoproteins may be obtained by applying all the lectins to a single blot.

Keywords

Platelet glycoproteins - Two-dimensional O’Farrell gel electrophoresis - Western blot - Avidin-biotin - Lectins

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References

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