Download PDF
Thromb Haemost 1978; 39(02): 366-378
DOI: 10.1055/s-0038-1646697
Original Article
Schattauer GmbH Stuttgart

Relationships of Adenine Nucleotide Metabolism to Platelet-Collagen Adhesion

Robert J Morin
The Department of Pathology, Harbor General Hospital, UCLA School of Medicine, Torrance, California 90509, U.S.A.
,
Albert F T Chen
The Department of Pathology, Harbor General Hospital, UCLA School of Medicine, Torrance, California 90509, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 20 January 1977

Accepted 12 August 1977

Publication Date:
12 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

Adhesion of platelets to collagen fibrils in a stirred system was inhibited by preincubation of platelets with combinations of 2-deoxy-D-glucose and oligomycin or antimycin. The inhibition of adhesion was associated with a decrease in metabolic ATP to 6% of control levels. Without metabolic inhibitors, platelets adherent to collagen fibrils were found to have catabolized approximately 57% of their metabolic ATP, and converted a major part of this to IMP. Storage pool ATP and ADP contents were also diminished in the adherent platelets. Pretreatment with imipramine resulted in 76% inhibition of the release reaction, but only 5% inhibition of adhesion. Imipramine-treated platelets that were adherent to collagen showed significant depletion of metabolic ATP, but markedly diminished conversion of ATP to IMP as compared to control adherent platelets. Inhibition of deamination of platelet AMP by coformycin or erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) did not inhibit adhesion, although platelets adherent to collagen after treatment with these agents showed depletion of metabolic ATP. These studies suggest that adhesion is an energy dependent process, occurring independently of release, and not associated with conversion of ATP to IMP. The energy dependent portions of the adhesion process are probably disc to sphere transformation and pseudopod formation, the ATP threshold requirement is relatively low, and the ATP utilized can probably be regenerated during the adhesion process via glycolysis and oxidative phosphorylation.

 

  • References

  • 1 Agarwal KC, Parks RE. 1975; Adenosine analogs and human platelets. Effects on nucleotide pools and the aggregation phenomenon. Biochemical Pharmacology 24: 2239
    CrossrefPubMedGoogle Scholar
  • 2 Agarwal KC, Parks Jr RE. 1977; Inhibition of muscle 5’ adenosine monophosphate deaminase by the nucleoside antibiotics coformycin and deoxycoformycin. Biochemical Pharmacology 26: 663
    CrossrefPubMedGoogle Scholar
  • 3 Barber AJ, Jamieson GA. 1971; Platelet collagen adhesion: characterization of collagen gluco-syltransferase of plasma membranes of human blood platelets. Biochimica et biophysica acta 252: 533
    CrossrefPubMedGoogle Scholar
  • 4 Baumgartner HR. 1973. The subendothelial surface and thrombosis. Proceedings of the Fourth Congress of the International Society on Thrombosis and Haemostasis; Vienna, Austria: 1973. p 42
    Google Scholar
  • 5 Bergman I, Loxley R. 1963; Two improved and simplified methods for the spectrophotometric determination of hydroxyproline. Analytical Chemistry 35: 1961
    CrossrefPubMedGoogle Scholar
  • 6 Brass LF, Faile D, Bensusan HB. 1976; Direct measurement of the platelet collagen interaction by affinity chromatography on collagen/sepharose. The Journal of Laboratory and Clinical Medicine 87: 525
    PubMedGoogle Scholar
  • 7 Cazenave JP, Packham M, Guccione MA, Mustard JF. 1974; Inhibition of platelet adherence to a collagen-coated surface by agents that inhibit platelet shape change and clot retraction. The Journal of Laboratory and Clinical Medicine 84: 483
    PubMedGoogle Scholar
  • 8 Cazenave JP, Packham MA, Mustard JF. 1973; Adherence of platelets to a collagen-coated surface: development of a quantitative method. Journal of Laboratory and Clinical Medicine 82: 978
    PubMedGoogle Scholar
  • 9 Durham ACH. 1974; A unified theory of the control of actin and myosin in non-muscle movements. Cell 2: 123
    CrossrefPubMedGoogle Scholar
  • 10 Fischer D, Van Der Weyden MB, Snyderman R, Kelly WN. 1976; A role for adenosine deaminase in human monocyte maturation. The Journal of Clinical Investigation 58: 399
    CrossrefPubMedGoogle Scholar
  • 11 Hattori A, Tsukada T, Ito S, Koike K, Matsuoka M. 1974; Scanning electron microscope study of platelet “adhesiveness” to glass beads (Hellem II method). Normal subjects, the effect of aspirin and patients with von Willebrand’s disease Thrombosis et Diathesis Haemorrhagica Suppl (Suppl. 60) 447
    PubMedGoogle Scholar
  • 12 Holmsen H. 1975. Biochemistry of the platelet release reaction. In: Biochemistry and Pharmacology of Platelets CIBA Foundation Symposium 35; Elsevier, Amsterdam: pp 175-205
    Google Scholar
  • 13 Holmsen H, Day HJ. 1971; Adenine nucleotides and platelet function. Seminars in Haematology IV: 28
    PubMedGoogle Scholar
  • 14 Holmsen PH, Day HJ, Stormorken H. 1969; The blood platelet release reaction. Scandinavian Journal of Hematology Suppl 8 3
    PubMedGoogle Scholar
  • 15 Holmsen H, Day HJ, Storm E. 1969; Adenine nucleotide metabolism of blood platelets VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction. Biochimica et biophysica acta 186: 254
    CrossrefPubMedGoogle Scholar
  • 16 Holmsen H, Holmsen I, Bernhardsen A. 1966; Microdetermination of adenosine diphosphate and adenosine triphosphate in plasma with the firefly luciferase system. Analytical Biochemistry 17: 456
    CrossrefPubMedGoogle Scholar
  • 17 Holmsen H, Setkowsky CA, Day HJ. 1974; Effects of antimycin and 2-deoxyglucose on adenine nucleotides in human platelets. Biochemical Journal 144: 385
    CrossrefPubMedGoogle Scholar
  • 18 Holmsen H, Weiss HJ. 1970; Hereditary defect in the platelet release reaction caused by a deficiency in the storage pool of platelet adenine nucleotides. British Journal of Haematology 19: 643
    CrossrefPubMedGoogle Scholar
  • 19 Kattlove HE. 1974; Platelet ATP in ADP-induced aggregation. American Journal of Physiology 226: 325
    PubMedGoogle Scholar
  • 20 Kattlove HE. 1974; Primary platelet aggregation energy metabolism and the effect of inhibitors. Biochimica et biophysica acta 372: 135
    CrossrefPubMedGoogle Scholar
  • 21 Kattlove H, Gomez MH. 1975; Collagen-induced platelet aggregation: The role of adenine nucleotides and the release reaction. Thrombosis et Diathesis Haemorrhagica 34: 795
    PubMedGoogle Scholar
  • 22 Lee RG, Kim SW. 1976; Cyclic AMP and platelet adhesion to silastic surfaces. Thrombosis Research 3: 111
    PubMedGoogle Scholar
  • 23 Lyman B, Rosenberg L, Karpatkin S. 1971; Biochemical and biophysical aspects of human platelet adhesion to collagen fibers. Journal of Clinical Investigation 50: 1854
    CrossrefPubMedGoogle Scholar
  • 24 Mcpherson J, Zucker MB. 1976; Platelet retention in glass bead columns: Adhesion to glass and subsequent platelet-platelet interactions. Blood 47: 55
    PubMedGoogle Scholar
  • 25 Mcpherson VJ, Zucker MB, Friedberg NM, Rifkin PL. 1974; Platelet retention in glass bead columns: Further evidence for the importance of ADP. Blood 44: 411
    PubMedGoogle Scholar
  • 26 Meyer D. 1972; In vitro platelet adhesiveness. Methods of study and clinical significance. Advances in Experimental Medicine and Biology 34: 123
    PubMedGoogle Scholar
  • 27 Mohammad SF, Hardison MD, Glenn CH, Morton BD, Bolan JC, Mason RG. 1974; Adhesion of human blood platelets to glass and polymer surfaces. Haemostasis 3: 257
    PubMedGoogle Scholar
  • 28 Mohammad SF, Mason RG. 1974; Inhibition of human platelet-collagen adhesion reaction by amitryptiline and imipramine. Proceedings of the Society for Experimental Biology and Medicine 145: 1106
    PubMedGoogle Scholar
  • 29 MüRER EH. 1969; Clot retraction and energy metabolism of platelets. Effect and mechanism of inhibitors. Biochimica et biophysica acta 172: 266
    CrossrefPubMedGoogle Scholar
  • 30 Mürer EH, Hellem AJ, Rozenberg MC. 1967; Energy metabolism and platelet function. Scandinavian Journal of Clinical and Laboratory Investigation 19: 280
    CrossrefPubMedGoogle Scholar
  • 31 Puszkin S, Puszkin E, Katz AM, Aledort LM. 1974; Control of platelet actomyosin activity: Effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin. Biochimica et biophysica acta 347: 102
    CrossrefPubMedGoogle Scholar
  • 32 Reimers HJ, Kinlough-Rathbone RL, Cazenave JP, Senyi AF, Hirsh J, Packham A, Mustard JF. 1976; In vitro and in vivo functions of thrombin-treated platelets. Thrombosis and Haemostasis 35: 151
    Article in Thieme ConnectPubMedGoogle Scholar
  • 33 Ross R, Glomset JA. 1976; The Pathogenesis of Atherosclerosis. The New England Journal of Medicine 295: 369
    CrossrefPubMedGoogle Scholar
  • 34 Spaet TH, Lejnieks I. 1969; A technique estimation of platelet-collagen adhesion. Proceedings of the Society for Experimental Biology and Medicine 132: 1038
    PubMedGoogle Scholar
  • 35 Tschopp THB, Baumgartner HR. 1976; Enzymatic removal of ADP from plasma: Unaltered platelet adhesion but reduced aggregation on subendothelium and collagen fibrils. Thrombosis and Haemostasis 35: 334
    Article in Thieme ConnectPubMedGoogle Scholar
  • 36 Zucker-Franklin D, Nachman RL, Marcus AJ. 1967; Ultrastructure of thrombosthenin, the contractile protein of human blood platelets. Science 157: 945
    CrossrefPubMedGoogle Scholar