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Thromb Haemost 1979; 41(04): 796-803
DOI: 10.1055/s-0038-1646839
Original Articles
Schattauer GmbH Stuttgart

Immunohistochemical and Histochemical Investigations on In Vivo Thrombolysis with Urokinase in Rabbits

Shuusuke Hisano
The First Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Katsuo Sueishi
The First Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Yutaka Ishii
The First Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Kenzo Tanaka
The First Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
› Author Affiliations
Further Information

Publication History

Received 30 April 1977

Accepted 27 July 1978

Publication Date:
09 July 2018 (online)

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Summary

Using human urokinase in vivo thrombolysis was studied in autologous artificial thrombi in the pulmonary circulation of rabbits by immunofluorescence and Todd’s fibrinolysis autography techniques.

As compared to the control thrombi of the untreated rabbits, a small increase of thrombolysis was found in the rabbits treated with urokinase.

Urokinase fluorescence was observed in the leukocytes, but not along the fibrin fibrils in the thrombi of the rabbits treated with urokinase. By Todd’s fibrinolysis autography, lytic areas were observed around aggregated leukocytes in the thrombi of the rabbits treated with urokinase.

The small lysis of autologous artificial thrombi of rabbits by human urokinase may be caused by (1) low affinity of human urokinase for rabbit plasminogen, (2) weak adsorption of human urokinase to rabbit fibrin, (3) phagocytosis of human urokinase by leukocytes and (4) high level of antifibrinolytic activity of rabbit plasma.

 

  • References

  • 1 Blix S. 1969; The control of plasminogen and plasmin during thrombolytic therapy. Acta Medica Scandinavica 186: 479
    PubMedSearch in Google Scholar
  • 2 Chandler AB. 1958; In vitro thrombotic coagulation of the blood. Laboratory Investigation 7: 110
    PubMedSearch in Google Scholar
  • 3 Fletcher AP, Alkjaersig N, Sherry S, Genton E, Hirsh J, Bachmann F. 1965; The development of urokinase as a thrombolytic agent. Maintenance of a sustained thrombolytic state in man by its intravenous infusion Journal of Laboratory and Clinical Medicine 65: 713
    PubMedSearch in Google Scholar
  • 4 Genton E, Wolf PS. 1967; Experimental pulmonary embolism. Effect of urokinase therapy on organizing thrombi Journal of Laboratory and Clinical Medicine 70: 311
    PubMedSearch in Google Scholar
  • 5 Gottlob R, Nashef EL, Donas P, Piza F, Kolb R. 1973; Studies on thrombolysis with streptokinase. IV. Immunofluorescent investigation on the fibrin pattern and the content of plasminogen and plasma-plasmin-inhibitors in clots and thrombi of various age Thrombosis et Diathesis Haemorrhagica 29: 393
    PubMedSearch in Google Scholar
  • 6 Guest MM, Daly BM, Ware AG, Seegers WH. 1948; A study of antifibrinolysis activity in the plasmas of various animal species. Journal of Clinical Investigation 27: 785
    CrossrefPubMedSearch in Google Scholar
  • 7 Hamashima Y, Kyogoku M. 1968. Immunohistology. Igakushion Ltd.,; Tokyo, Japan:
    Search in Google Scholar
  • 8 Hedlin AM, Monkhouse FC, Milojevic SM. 1972; A comparative study of fibrinolytic activity in human, rat, rabbit, and dog blood. Canadian Journal of Physiology and Pharmacology 50: 11
    CrossrefPubMedSearch in Google Scholar
  • 9 Henry RL. 1965; Leukocytes and thrombosis. Thrombosis et Diathesis Haemorrhagica 13: 35
    PubMedSearch in Google Scholar
  • 10 Hisano S. 1978; Immunofluorescent study on thrombolysis with special reference to the patterns of distribution of the contents of fibrin, plasminogen, α2-macroglobulin and urokinase in artificial thrombi. Thrombosis and Hemostasis 39: 53
    PubMedSearch in Google Scholar
  • 11 Irfan M. 1968; Fibrinolytic activity in animals of different species. Quarterly Journal of Experimental Physiology 53: 373
    PubMedSearch in Google Scholar
  • 12 Koga M. 1971; Experimental study on resolution and organization of thrombi in pulmonary arteries of rabbits. Fukuoka Acta Medica 62: 525
    PubMedSearch in Google Scholar
  • 13 Kopitar M, Stegnar M, Accetto B, Lebez D. 1974; Isolation and characterization of plasminogen activator from pig leucocytes. Thrombosis et Diathesis Haemorrhagica 31: 72
    PubMedSearch in Google Scholar
  • 14 Maki M, Nagayama M, Endo T. 1975; Influence of urokinase on experimentally-induced DIC. Journal of Medical Enzymology 1: 496
    PubMedSearch in Google Scholar
  • 15 Mohler SR, Celander DR, Guest MM. 1958; Distribution of urokinase among the common mammals. American Journal of Physiology 192: 186
    PubMedSearch in Google Scholar
  • 16 Mustard JE, Kinlough-Rathbone RL, Packham MA. 1974; Recent status of research in the pathogenesis of thrombosis. Thrombosis et Diathesis Haemorrhagica Suppl 39: 157
    PubMedSearch in Google Scholar
  • 17 Nowak A, Gurewich V. 1974; Thrombolysis with streptokinase in rabbits. Does response, fibrin-clot specificity and laboratory evaluation of fibrinolytic effect Thrombosis et Diathesis Haemorrhagica 31: 265
    PubMedSearch in Google Scholar
  • 18 Prolopowicz J, Stormoken H. 1968; Fibrinolytic activity of leukocytes in smears of bone marrow and peripheral blood. Scandinavian Journal of Hematology 5: 129
    PubMedSearch in Google Scholar
  • 19 Sakuragawa N, Takahashi K, Matsuoka M. 1975; Studies on thrombolytic therapy using urokinase. Journal of Medical Enzymology 1: 518
    PubMedSearch in Google Scholar
  • 20 Sawyer WD, Alkjaersig N, Fletcher AP, Sherry S. 1961; Thrombolytic therapy. Archives of Internal Medicine 107: 21A
    PubMedSearch in Google Scholar
  • 21 Tanaka K. 1968; Thrombosis. Fukuoka Acta Medica 59: 337
    PubMedSearch in Google Scholar
  • 22 Todd AS, Nunn A. 1967. Fibrinolytic activity in tissue and thrombi. In Tissue Factors in the Haemostasis of the Coagulation-Fibrinolysis System, Unione Chimica Medicamenta Turin: pp 57
    Search in Google Scholar
  • 23 Ueno T, Takeuchi T, Maekawa T. 1975; Studies on in vivo metabolism of I125-urokinase with special reference to its thrombolytic action. Journal of Medical Enzymology 1: 340
    PubMedSearch in Google Scholar