Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

R Schneppenheim; H Plendl; U Budde

doi:10.1055/s-0038-1647016

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1988; 60(02): 133-136
DOI: 10.1055/s-0038-1647016

Original Article

Schattauer GmbH Stuttgart

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

R Schneppenheim

^*The Department of Pediatrics, Klinikum der Christian-Albrechts-Universität Kiel, Hamburg, FRG

,

H Plendl

^**The Department of Human Genetics, Klinikum der Christian-Albrechts-Universität Kiel, Hamburg, FRG

,

U Budde

^***The General Hospital Harburg, Hamburg, FRG

› Author Affiliations

Abstract

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Summary

A luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

Keywords

Von Willebrand factor (vWF) - Multimers - Immunoblotting - Peroxidase-linked antibodies - Luminography

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References

References
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