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Thromb Haemost 1990; 64(01): 021-025
DOI: 10.1055/s-0038-1647147
Original Article
Schattauer GmbH Stuttgart

Clinical Significance of Various ELISA Assays for Detecting Antiphospholipid Antibodies[*]

C R Falcón
The Haemostasis and Thrombosis Section, Division of Haematology, Hospital de Clinicas, University of Buenos Aires, Argentina
,
A M Hoffer
The Haemostasis and Thrombosis Section, Division of Haematology, Hospital de Clinicas, University of Buenos Aires, Argentina
,
R R Forastiera
The Haemostasis and Thrombosis Section, Division of Haematology, Hospital de Clinicas, University of Buenos Aires, Argentina
,
L O Carreras
The Haemostasis and Thrombosis Section, Division of Haematology, Hospital de Clinicas, University of Buenos Aires, Argentina
› Author Affiliations
Further Information

Publication History

Received 08 September 1989

Accepted after revision 12 March 1990

Publication Date:
25 July 2018 (online)

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Summary

We have compared the prevalence of antiphospholipid antibodies (APA) measured by enzyme-linked immunosorbent assay (ELISA), in 119 selected patients using five different antigens: bovine cardiolipin, phosphatidylserine, phosphatidylinositol, bovine partial thromboplastin and human brain partial thromboplastin. All the plasmas have been evaluated for the presence of lupus anticoagulant (LA) activity by clotting techniques. We found a significant association between the incidence of LA and APA (p <0.001), only moderate agreement between the prolongation of the activated partial thromboplastin time (APTT) and ELISAs (r around 0.50) and a good agreement between the ELISAs (r around 0.80). The combination of antibodies against cardiolipin (ACA) and human brain partial thromboplastin (AHPTA) allowed the detection of antibodies in most of the LA positive cases. ACA, AHPTA and antiphosphatidylinositol antibodies detected all the positive samples. Six patients (5%) had a single APA detected. The clinical associations of APA according to phospholipid specificity, immunoglobulin isotype and titer are shown.

Dedicated to Professor Marc Verstraete on the occasion of his 65th birthday.


 

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