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Thromb Haemost 1990; 64(01): 145-149
DOI: 10.1055/s-0038-1647271
Original Article
Schattauer GmbH Stuttgart

Binding of Plasma von Wllebrand Factor by Arterial Microfibrils

Françoise Fauvel-Lafève
The Unité 150 INSERM, Vaisseaux et Hômostase, URA 334 CNRS, Hôpital Saint-Louis
,
Yves J Legrand
*   Hôpital Lariboisíère, Paris, France
› Author Affiliations
Further Information

Publication History

Received 06 October 1989

Accepted after revision 19 March 1990

Publication Date:
25 July 2018 (online)

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Summary

We developed an ELISA test to measure the binding of plasma von Willebrand factor (vWF) to arterial microfibrils and compared this interaction to the binding of vWF to collagen under the same conditions. We found that vWF binds to microfibrils in a similar manner as it binds to collagen: the binding was independent of the presence of cations, and temperature of incubation and was displaced by 1 M NaCl. Using purified 125I-vwF we showed that the binding was saturable and could be displaced by cold vWF in excess. Using immunoblotting we showed that vWF binds to a 97 kDa protein present in the microfibrils different from the 128 kDa thrombospondin-like structure (GP 128) which in microfibrils is known to interact with blood platelets. These results indicate that in the subendothelium, microfibrils bind plasma vWF and this reinforces the thrombogenic role of these structures.

 

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