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Thromb Haemost 1990; 64(02): 294-296
DOI: 10.1055/s-0038-1647304
Original Article
Schattauer GmbH Stuttgart

Venostasis but not DDAVP Infusion Provokes the Plasma Fibronectin Increase

M Letowska
The Department of Blood Storage and Preparation, Institute of Hematology, Warsaw, Poland
,
K Bykowska
1   The Laboratory of Blood Coagulation and Hemostasis, Institute of Hematology, Warsaw, Poland
,
J Sablinski
The Department of Blood Storage and Preparation, Institute of Hematology, Warsaw, Poland
,
S Lopaciuk
1   The Laboratory of Blood Coagulation and Hemostasis, Institute of Hematology, Warsaw, Poland
,
M Kopeć
1   The Laboratory of Blood Coagulation and Hemostasis, Institute of Hematology, Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Received 22 September 1989

Accepted after revision11 May 1990

Publication Date:
25 July 2018 (online)

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Summary

Plasma fibronectin (pFN), von Willebrand factor antigen (vWf: Ag), factor VIII procoagulant activity, fibrinogen, euglobulin lysis time (ELT) and hematocrit were determined in healthy blood donors before and after venostasis as well as after intravenous infusion of l-deamino-8-D-arginine vasopressin (DDAVP). Both venostasis and DDAVP provoked an increase in vWf : Ag and shortening in the ELT. In contrast, venostasis only but not DDAVP induced an increase in pFN levels which was statistically significant with and without correction for a concomitant hematocrit increment. The results indicate that there is a distinct difference in the patterns of venostasis and DDAVP mediated release of proteins from the vessel wall.

 

  • References

  • 1 Jaffe EA, Hoyer LW, Nachman RL. Synthesis of antihaemophilic factor antigen by cultured human endothelial cells. J Clin Invest 1973; 52: 2757-2764
    CrossrefPubMedGoogle Scholar
  • 2 Verweij CL. Biosynthesis of human von Willebrand factor. Haemostasis 1988; 18: 224-245
    PubMedGoogle Scholar
  • 3 Van Hinsbergh VW M. Regulation of the synthesis and secretion of plasminogen activators by endothelial cells. Haemostasis 1988; 18: 307-327
    PubMedGoogle Scholar
  • 4 Jaffe EA, Mosher DF. Synthesis of fibronectin by cultured endothelial cells. J Exp Med 1978; 147: 1779-1791
    CrossrefPubMedGoogle Scholar
  • 5 Nilsson IM, Vilhardt H, Holmberg L, Astedt B. Association between factor VIII related antigen and plasminogen activator. Acta Med Scand 1982; 211: 105-112
    PubMedGoogle Scholar
  • 6 Schwarz HP, Luger A, Graf H, Stummvoll HK, Aiginger P. Fibronectin and venostasis. Thromb Haemostas 1982; 48: 345
    PubMedGoogle Scholar
  • 7 Ciemiewski CS, Wilczynska M, Augustyniak W, Kowalska MA. Elevated plasma fibronectin during venous occlusion. Thromb Haemostas 1985; 53: 157
    PubMedGoogle Scholar
  • 8 Musso R, Longo A, Cacciola RR, Lombardo A, Giustolisi R, Cacciola E. Elevated fibronectin plasma levels in diabetes mellitus are expression of increased synthesis and release by vascular endothelium. Thromb Haemostas 1989; 61: 150-151
    PubMedGoogle Scholar
  • 9 Mannucci PM, Remuzzi G, Pusineri F, Lombardi R, Valsecchi C, Mecca G, Zimmerman TS. Deamino-8-D-arginine vasopressin shortens the bleeding time in uremia. N Engl J Med 1983; 308: 8-12
    CrossrefPubMedGoogle Scholar
  • 10 Moffat EH, Giddings JC, Bloom AL. The effect of desamino-D-arginine vasopressin (DDAVP) and naloxone infusions on factor VIII and possible endothelial cell (EC) related activities. Br J Haematol 1984; 57: 651-661
    CrossrefPubMedGoogle Scholar
  • 11 Saba TM, Blumenstock FA, Evanega G, Staehler F, Cho E. Evaluation of rapid immunoturbidimetric assay for opsonic fibronec-tin in surgical and trauma patients. J Lab Clin Med 1981; 98: 482-491
    PubMedGoogle Scholar
  • 12 Bykowska K, Wegrzynowicz Z, Lopaciuk S, Kopeć M. Effect of proteolysis on quantitation of plasma fibronectin concentration by two immunoassays (electroimmunoassay and immunoturbidimetric technique). Thromb Haemostas 1985; 53: 377-380
    PubMedGoogle Scholar
  • 13 Miekka SI, Ingham KC, Menache D. Rapid methods for isolation of human plasma fibronectin. Thromb Res 1982; 27: 1-14
    CrossrefPubMedGoogle Scholar
  • 14 Proctor RR, Rapaport SI. The partial thromboplastin time with kaolin. A simple screening test for first stage plasma clotting deficiencies. Am J Glin Pathol 1961; 36: 212-219
    PubMedGoogle Scholar
  • 15 Laurell CB. Electro-immunoassay. Scand J Clin Lab Invest 1972; (Suppl. 29) suppl 124: 21-37
    PubMedGoogle Scholar
  • 16 Bidwell E. Fibrinolysins in human plasma. Biochem J 1953; 55: 497-506
    CrossrefPubMedGoogle Scholar
  • 17 Kowarzyk H, Buluk K. Postepy badan nad krzepnieciem krwi. Post Hig Med Dosw 1950; 2: 1-76
    PubMedGoogle Scholar
  • 18 Bichet DG, Razi M, Lonergan M, Arthus MF, Papukna V, Kortas C, Baijon JN. Hemodynamic and coagulation responses to 1-desamino (8-D-arginine) vasopressin in patients with congenital nephrogenic diabetes insipidus. N Engl J Med 1988; 318: 881-887
    CrossrefPubMedGoogle Scholar