Download PDF
Thromb Haemost 1990; 64(03): 445-449
DOI: 10.1055/s-0038-1647334
Original Article
Schattauer GmbH Stuttgart

Effect of Soluble and Immobilized Plasmin on Fibrinogen and Platelets

F Senatore
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
H Shankar
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
C H Ho
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
S Avantsa
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
M Allon
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
R Posteraro
1   The Department of Radiology, Texas Tech University Health Sciences Center, Lubbock, Texas, USA
› Author Affiliations
Further Information

Publication History

Received: 10 August 1989

Accepted after revision27 June 1989

Publication Date:
25 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

Plasmin was immobilized on collageno-elastic tubes (CET) using carbodiimide as the cross-linking agent. The effects of plasmin-CET grafts and corresponding soluble plasmin on fibrinogen, thrombin-mediated fibrinogen activation, and platelet activity, were investigated

There was a significant increase in fibrinogen deposition on plasmin-CETs over non-plasmin (i.e. control) CETs. Furthermore, exposure of fibrinogen to plasmin CETs enhances its deposition to control grafts situated downstream. Plasmin-bound CETs retained higher platelet deposition when preliminarily coated with fibrinogen. Finally, plasmin exerted a positive effect on thrombin-mediated fibrinogen activation at low plasmin concentrations. A mechanistic hypothesis aimed at interpreting this finding is proposed.

 

  • References

  • 1 Senatore E, Shankar H, Venkataramani ES. Development of biocompatible vascular prostheses. CRC Crit Rev Biocompatibility 1988; 4: 281-340
    PubMedGoogle Scholar
  • 2 Rosenberg N, Lord GH, Hendersen J, Bothwell Jw. Collagen arterial graft of bovine origin. Surgery 1970; 67: 951-6
    PubMedGoogle Scholar
  • 3 Parsonnet V, Tiro AC, Brief DK, Alpert J, Brener BJ. The fibrocollagenous tube as a small arterial prosthesis. In: Graft Materials in Vascular Surgery Dardik H. ed Symposia Specialists; Miami: 1978: 249-262
  • 4 Dardike H, Ibrahim IM, Sussman B, Kahn M, Dardik I. Glutaraldehyde-tanned human umbilical vein grafts. In: Biologic and Synthetik Vascular Prostheses Stanley JC. ed Grune & Stratton; New York: 1982: 445-466
  • 5 Berger K, Sauvage LR, Rao AM, Wood SJ. Healing of arterial prostheses in man: Its incompleteness. Ann Surg 1972; 175: 118-126
    CrossrefPubMedGoogle Scholar
  • 6 Sugitachi A, Thkagi K, Inaska S, Kosaki G. Immobilization of plasminogen activator, urokinase on nylon. Thromb Haemostas 1978; 39: 426-446
    PubMedGoogle Scholar
  • 7 Senatore FE, Bernat FR. Urokinase bound to fibrocollagenous tubes: An in vitro kinetic study. Biotechnol Bioeng 1986; 28: 58-63
    CrossrefPubMedGoogle Scholar
  • 8 Senatore FE, Bernath FR, Meisner K. Clinical study of urokinasebound fibrocollagenous tubes. J Biomed Mater Res 1986; 20: 177-188
    CrossrefPubMedGoogle Scholar
  • 9 Senatore FE, Bernath FR. Lysis time and FDP immunoprecipitation by soluble and immobilized urokinase. J Biomed Mater Res 1986; 20: 189-204
    CrossrefPubMedGoogle Scholar
  • 10 Watanabe S, Shimizu Y, Teramatsu T, Murachi T, Hiro T. The in vitro and in vivo behavior of urokinase immobilized onto collagensynthetic polymer composite material. J Biomed Mater Res 1981; 15: 553
    CrossrefPubMedGoogle Scholar
  • 11 Rimon A, Rimon S. Immobilized components of the plasmin system. In: Insolubilized Enzymes Salmona M, Saronio C, Garattini S. eds Raven Press; New York: 1974
  • 12 Shankar H, Senatore F, Zuniga P, Venkataramani ES. Enhanced in vitro fibrinolytic activity of immobilued plasmin on collagen beads. J Biomed Mater Res 1988; 21: 897-912
    PubMedGoogle Scholar
  • 13 Adams GA, Feuerstein IA. How much fibrinogen or fibronectin is enough for platelet adhesion. Tians Am Soc Artif Intern Organs 1981; 27: 219-224
    PubMedGoogle Scholar
  • 14 Young BR, Lambrecht LK, Cooper SL, Mosher DE. plasma proteins: Their role in initiating platelet and fibrin deposition on biomaterials. In: Biomaterials: Interfacial Phenomena and Applications, Adv Chem Ser No. 199 Cooper SL, peppas NA. eds American Chemical Society; Washington DC: 1982: 317-350
  • 15 Jayakumari N. Blood material interaction. Biomat Med Dev Artif Organs 1985; 12: 97-112
    PubMedGoogle Scholar
  • 16 Pitt WG, Park K, Cooper SL. Sequential protein adsorption and thrombus deposition on polymeric biomaterials. J Colloid and Interface Sci 1986; 111: 343-362
    CrossrefPubMedGoogle Scholar
  • 17 Abrahamsen AE A. modification of the technique for Cr-51 labelling of blood platelets. Scand J Haematol 1968; 5: 53-63
    PubMedGoogle Scholar
  • 18 Carr ME, Hermans J. Size and density of fibrin fibers from turbidity. Macromolecules 1978; 11: 46-50
    CrossrefPubMedGoogle Scholar
  • 19 Francis CW, Marder VJ. Physiologic regulation and pathologic disorders of fibrinolysis. In: Haemostasis and Thrombosis: Basic Principles and Clinical Practice Colman RW, Hirsh J, Marder VJ, salzman E. eds J B Lippincott; Philadelphia: 1987: 358-379
  • 20 Pizzo SV, Schwartz ML, Hill RL, McKee PA. The effect of plasmin on the subunit structure of human fibrinogen. J Biol Chem 1972; 247: 636-45
    PubMedGoogle Scholar
  • 21 Medved LV, Gorkun OV, Manyakov VI, Belitser VA. The role of fibrinogen crC-domain in the fibrin assembly process. FEBS Letters 1985; 181: 109-112
    CrossrefPubMedGoogle Scholar
  • 22 Medved LY, Gorkun OV, Manyakov VE, Belitser VA. crC-domain of fibrinogen as a structure accelerating the autoassembly of fibrin. Mol Biol 1986; 20: 366-375
    PubMedGoogle Scholar
  • 23 Venkataramani ES, Senatore E, Feola M, Tran RM. Nonthrombogenic small-caliber human umbilical vein vascular prosthesis. Surgery 1986; 99: 735-42
    PubMedGoogle Scholar
  • 24 Sakata Y, Mimuro J, Aoki N. Differential binding of plasminogen to crosslinked and non-crosslinked fibrins: Its significance in hemostatic defect in factor XIII deficiency. Blood 1984; 63 (06) 1393-1401
    PubMedGoogle Scholar
  • 25 Shankar H, Senatore E, Zuniga P, Venkataramani E. Enhanced Invitro fibrinolytic activity of immobilized plasmin on collagen beads. J Biomed Mater Res 1987; 21: 897-912
    CrossrefPubMedGoogle Scholar