Thromb Haemost 1990; 64(03): 445-449
DOI: 10.1055/s-0038-1647334
Original Article
Schattauer GmbH Stuttgart

Effect of Soluble and Immobilized Plasmin on Fibrinogen and Platelets

F Senatore
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
H Shankar
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
C H Ho
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
S Avantsa
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
M Allon
The Department of Chemical Engineering, Texas Tech University, Lubbock, Texas, USA
,
R Posteraro
1  The Department of Radiology, Texas Tech University Health Sciences Center, Lubbock, Texas, USA
› Author Affiliations
Further Information

Publication History

Received: 10 August 1989

Accepted after revision27 June 1989

Publication Date:
25 July 2018 (online)

Summary

Plasmin was immobilized on collageno-elastic tubes (CET) using carbodiimide as the cross-linking agent. The effects of plasmin-CET grafts and corresponding soluble plasmin on fibrinogen, thrombin-mediated fibrinogen activation, and platelet activity, were investigated

There was a significant increase in fibrinogen deposition on plasmin-CETs over non-plasmin (i.e. control) CETs. Furthermore, exposure of fibrinogen to plasmin CETs enhances its deposition to control grafts situated downstream. Plasmin-bound CETs retained higher platelet deposition when preliminarily coated with fibrinogen. Finally, plasmin exerted a positive effect on thrombin-mediated fibrinogen activation at low plasmin concentrations. A mechanistic hypothesis aimed at interpreting this finding is proposed.