Thromb Haemost 1990; 64(03): 469-472
DOI: 10.1055/s-0038-1647338
Original Article
Schattauer GmbH Stuttgart

Identification of Platelet Antigens by Immunoprecipitation of Unlabelled Platelet Glycoproteins

Werner Stenziger
The Department of Internal Medicine, University of Münster, Federal Republic of Germany
,
Beate Kehrel
The Department of Internal Medicine, University of Münster, Federal Republic of Germany
,
Jürgen van de Loo
The Department of Internal Medicine, University of Münster, Federal Republic of Germany
› Author Affiliations
Further Information

Publication History

Received: 15 November 1989

Accepted after revision02 June 1990

Publication Date:
25 July 2018 (online)

Summary

A time-saving and sensitive method for the identification of antigens on unlabelled platelet glycoproteins (GP) based on immunoprecipitation has been developed. Platelet solubilisates were incubated with antibodies to form GP-antibody complexes that were precipitated with Protein G Sepharose 4 Fast Flow (PGS). Pcs-bound material was eluted, separated by SDSPAGE under reducing conditions and visualized by silver staining. The method was designed as an alternative to radioimmunoprecipitation for laboratories not equipped to work with radioactive substances. It is proposed as a complementary procedure for the characterization of platelet GP antigens by murine monoclonal antibodies and human alloantibodies.