Purification and Properties of Human Factor IXa

Katalin Váradi; Susan Elödi

doi:10.1055/s-0038-1648000

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 35(03): 576-585
DOI: 10.1055/s-0038-1648000

Original Article

Schattauer GmbH

Purification and Properties of Human Factor IX_a

Katalin Váradi

¹Department of Blood Coagulation, National Institute of Haematology and Blood Transfusion, Budapest, Hungary

,

Susan Elödi

¹Department of Blood Coagulation, National Institute of Haematology and Blood Transfusion, Budapest, Hungary

› Author Affiliations

Abstract

Summary

Human factor IX_a was purified 5,000-fold from serum by ion exchange chromatography. The preparation was free from other clotting factors. Both pH sensitivity and heat stability of purified factor IX_a appeared to be different from those of factor IX in the plasma. The molecular weight of human factor IX_a is 80,000 as estimated from gel-filtration experiments. Modification of seryl or histidyl side chains abolished the activity of factor IX_a.

Full Text

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