Some Observations on the Preparation of Platelet-Rich Plasma

Brian P. Woods; Angela Dennehy; Noel Clarke

doi:10.1055/s-0038-1648045

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1976; 36(02): 302-310
DOI: 10.1055/s-0038-1648045

Original Article

Schattauer GmbH

Some Observations on the Preparation of Platelet-Rich Plasma

Autoren

Brian P. Woods^*

¹Department of Pathology, University College, Earlsfort Terrace, Dublin 2, Ireland
Angela Dennehy

¹Department of Pathology, University College, Earlsfort Terrace, Dublin 2, Ireland
Noel Clarke

¹Department of Pathology, University College, Earlsfort Terrace, Dublin 2, Ireland

Abstract

Summary

In order to prepare platelet-rich plasma a constant height and volume of citrated whole blood was centrifuged at different gravity forces and times. The supernatant plasmas were examined for platelet concentrations, plasma volumes and optical densities. Results are described showing that gravity force and time have an important influence on the platelet numbers and the plasma volumes recovered.

The light attenuation by different concentrations of platelets in plasma, using light path lengths of 5 mm and 10 mm, was compared. The shorter light path provides greater linearity between optical density and platelet numbers especially at higher concentrations.

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Referenzen

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