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Thromb Haemost 1994; 72(06): 931-936
DOI: 10.1055/s-0038-1648986
Original Article
Schattauer GmbH Stuttgart

Detection of Plasminogen Activator Inhibitor-1 (PAI-1) mRNA in Human Megakaryocytes by In Situ Hybridization

M C Alessi
The Laboratory of Hematology, CHU Timone, Marseille, France
,
N Chomiki
The Laboratory of Hematology, CHU Timone, Marseille, France
,
R Berthier
The Laboratory of Hematology, CHU Timone, Marseille, France
,
A Schweitzer
1   Unité de Recherche sur I’Hémostase Cellulaire et Moléculaire, INSERM 217, Grenoble, France
,
C Fossat
1   Unité de Recherche sur I’Hémostase Cellulaire et Moléculaire, INSERM 217, Grenoble, France
,
I Juhan-Vague
The Laboratory of Hematology, CHU Timone, Marseille, France
› Author Affiliations
Further Information

Publication History

Received 12 April 1994

Accepted after resubmission 11 August 1994

Publication Date:
06 July 2018 (online)

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Summary

Platelets have been described to contain a large proportion of the circulating plasminogen activator inhibitor type 1 (PAI-1) which is released on platelet activation. This protein could be taken up by platelets from the plasma or synthesized by megakaryocytes (MKs). Recently, PAI-1 mRNA has been detected in a human megakaryoblastic leukemia cell line (MEG-01) by the polymerase chain reaction (PCR). However, a direct demonstration of its presence in normal human MKs is lacking.

In order to prove directly the megakaryocytic origin of platelet PAI-1, the MEG-01 cell line, human bone marrow enriched in MKs, and bone marrow smears from allogeneic bone marrow transplantation donors were investigated for the presence of PAI-1 mRNA using in situ hybridization (ISH). Specimens of bone marrow were first stained with May-Grunwald Giemsa (MGG) for cell identification according to their morphology. Subsequently, the same slides were used for ISH. PAI-1 mRNA was clearly demonstrated in the MEG-01 cell line and in MKs, and its presence correlated with the detection of PAI-1 antigen by immunocytochemistry. PAI-1 mRNA was also detected in morphologically characterized mature granulocytes of marrow samples.

 

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