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Thromb Haemost 1973; 30(02): 307-314
DOI: 10.1055/s-0038-1649079
Original Article
Schattauer GmbH

The Presence of Glycogen Synthase in Preparations of Platelet Plasma Membranes

J. H Greenberg
1   American National Red Cross Blood Research Laboratory, 9312 Old Georgetown Road, Bethesda, Maryland 20014
,
A. P Fletcher
1   American National Red Cross Blood Research Laboratory, 9312 Old Georgetown Road, Bethesda, Maryland 20014
,
G. A Jamieson
1   American National Red Cross Blood Research Laboratory, 9312 Old Georgetown Road, Bethesda, Maryland 20014
› Author Affiliations
Further Information

Publication History

Received for publication 22 March 1973

Accepted for publication 26 May 1973

Publication Date:
24 July 2018 (online)

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Summary

Glycogen synthase and glycogen (63 ± 35 μg/unit) have been identified in partially-purified preparations of platelet membranes isolated by density-step centrifugation following glycerol-lysis; this combination is responsible for the endogenous (collagen-independent) activity measured during the assay of collagen: glucosyltransferase. Glycogen synthesized in this way may be complexed with protein since it is precip-itable with trichloracetic acid. High and low density membranes prepared by continuous density gradient centrifugation are devoid of glycogen synthase activity.

 

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