Platelet Aggregation Induced by DAS in Vitro: Some Investigations on Its Mechanism of Action

K. U Benner; K. A Schumacher; H. G Classen

doi:10.1055/s-0038-1649170

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1974; 31(02): 354-362
DOI: 10.1055/s-0038-1649170

Original Article

Schattauer GmbH

Platelet Aggregation Induced by DAS in Vitro: Some Investigations on Its Mechanism of Action^[*]

K. U Benner

¹Institute of Normal and Pathological Physiology, University of Cologne, and Institute of Experimental Therapy, University of Freiburg, Germany

,

K. A Schumacher

¹Institute of Normal and Pathological Physiology, University of Cologne, and Institute of Experimental Therapy, University of Freiburg, Germany

,

H. G Classen

¹Institute of Normal and Pathological Physiology, University of Cologne, and Institute of Experimental Therapy, University of Freiburg, Germany

› Institutsangaben

Abstract

Summary

The effect of the depressor active substance (DAS) on platelets of men, cats, pigs, dogs, rats, and rabbits has been studied by the method of Born (1962). DAS was found to induce platelet aggregation only in human and feline platelet rich plasma (PRP). Nevertheless, there are some striking similarities between platelet aggregation induced by DAS and ADP (i.e. inhibition by the same compounds, such as adenosine, tosylarginine methylester, or p-chloromercuribenzoic acid). The species specifity and a marked tachyphylactic action on platelets of both species makes DAS clearly discernible from all the other aggregation inducing substances which have been studied so far. From additional experiments there is evidence that DAS acts on human and cat platelets via a release reaction of cellular substances known to enhance platelet aggregation in a second phase. This process is strongly dependent on the presence of Ca⁺⁺.

Volltext

Referenzen

References
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Platelet Aggregation Induced by DAS in Vitro: Some Investigations on Its Mechanism of Action[*]

Platelet Aggregation Induced by DAS in Vitro: Some Investigations on Its Mechanism of Action^[*]