The Half-Life of Plasmic Degradation Products of Human Fibrinogen in Rabbits

N Ardaillou; L Dray; A. Z Budzynski; V. J Marder; M. J Larrieu

doi:10.1055/s-0038-1649220

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 37(02): 201-209
DOI: 10.1055/s-0038-1649220

Original Article

Schattauer GmbH

The Half-Life of Plasmic Degradation Products of Human Fibrinogen in Rabbits

N Ardaillou

¹Department of Coagulation (U.143, I.N.S.E.R.M.), Institut de Pathologie Cellulaire, Hôpital de Bicêtre, 94270 Le Kremlin-Bicêtre, France

,

L Dray

¹Department of Coagulation (U.143, I.N.S.E.R.M.), Institut de Pathologie Cellulaire, Hôpital de Bicêtre, 94270 Le Kremlin-Bicêtre, France

,

A. Z Budzynski

^*Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania

,

V. J Marder

^*Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania

,

M. J Larrieu

¹Department of Coagulation (U.143, I.N.S.E.R.M.), Institut de Pathologie Cellulaire, Hôpital de Bicêtre, 94270 Le Kremlin-Bicêtre, France

› Author Affiliations

Abstract

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Summary

Human fibrinogen and purified plasmic degradation fragments X (stages 1 and 2), D and E were labelled with 125-1 using the lactoperoxidase method. The chromatographic, elec-trophoretic and immunologic properties of the labelled proteins were found to be similar to those of non-labelled fragments. All the degradation products diffused rapidly (T½ 0.27–0.75 hours) from the intravascular space of rabbits, as compared with fibrinogen (4.26 hours). In addition, the metabolic half-life was found to be 49.3 hours for fibrinogen, as compared with only 5.1, 6.1, 2.3 and 1.4 for fragments X (stage 1), X (stage 2), D and E, respectively. The metabolic half-life roughly reflects the molecular size of the degradation products.

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References

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