Binding of Collagen by Canine Blood Platelets

Summary

The binding of ¹²⁵I-collagen (tropocollagen) by canine blood platelets occurred in a concentration-dependent manner but no saturation effect could be observed. The binding of collagen was not entirely specific for platelets since various eucaryotic and procaryotic cells quantitatively bound collagen as well or better.

The temporal response to added collagen appeared to be binding, ³H-serotonin release, and finally platelet aggregation. Non-polymerizing salt-soluble tropocollagen was bound as well as acid-soluble tropocollagen, however neither ³H-serotonin release nor platelet aggregation occurred. Furthermore, the binding activity was not destroyed by treatment with collagenase, galactose oxidase and glucose oxidase, nor by periodate oxidation.

Platelet aggregation closely paralleled acid soluble collagen polymerization and both events were equally inhibited by arginine; however, arginine did not interfere with collagen binding. Scanning electron microscopy revealed an unusual morphological platelet response to collagen and platelets appeared to be nucleation sites for collagen polymerization.

• References

• 1 Baumgartner H. R. Platelet interaction with vascular structures. Thrombosis et Diathesis Haemorragica Supplements 1972; a 51: 161
• 2 Baumgartner H. R. Morphometric quantitation of adherence of platelets to an artificial surface and components of connective tissue. Thrombosis et Diathesis Haemorragica Supplement 1972; b 60: 39
• 3 Baumgartner H. R, Haudenschild C. Adhesion of platelets to subendothelium. Annals of the New York Academy of Science 1972; 201: 22
• 4 Born G. V. R. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (London) 1972; 194: 927
• 5 Bossman H. B. Platelet adhesiveness and aggregation: The collagen: glycosyl polypeptide: N-acetyl-galactosaminyl and glycoprotein: galactosyl transferases of human platelets. Biochemical and Biophysical Research Communications 1971; 43: 1118
• 6 Brass L. F, Bensusan H. P. The role of collagen quaternary structure in the platelet: collagen interaction. Journal of Clinical Investigation 1974; 54: 1480
• 7 Chesney C, Mci Harper E, Coleman R. W. Critical role of carbohydrate side chains of collagen in platelet aggregation. Journal of Clinical Investigation 1972; 51: 2693
• 8 Ehrman R. L, Gey G. D. The growth of cells on a transparent gel of reconstituted rat-tail collagen. Journal of the National Cancer Institute 1956; 16: 1375
• 9 Einbinder J, Schubert M. Binding of mucopolysaccharides and dyes by collagen. Journal of Biological Chemistry 1951; 188: 335
• 10 Faulk W. P, Conochie L. B, Temple A. Immunobiology of membrane-bound collagen on mouse fibroblasts. Nature (London) 1975; 256: 123
• 11 Gallop P.M, Seifter S. Preparation and properties of soluble collagens. In Kolowick S. J, Kaplan N. O. (ed.) Methods in Enzymology 1963; 6: 635-641.
• 12 Gordon J. L, Dingle J. T. Binding of radiolabeled collagen to blood platelets. Journal of Cell Science 1974; 16: 157
• 13 Gross J, Kirk D. The heat precipitation of collagen from neutral salt solutions: some rate-regulating factors. Journal of Biological Chemistry 1958; 233: 355
• 14 Harper E, Simons E. R, Chesney C, Mci Coleman R. W. The effect of chemical or enzymatic modifications upon the ability of collagen to form multimers and to initiate platelet aggregation. Thrombosis Research 1975; 7: 113
• 15 Harris R, Ukaejiofo E. O. Tissue typing using a routine one-step lymphocyte separation technique. British Journal of Haematology 1970; 18: 229
• 16 Hirsh J, Doery J. C. G. Platelet function in health and disease. Progress in Haematology 1971; 7: 185
• 17 Holmsen H. The platelet: its membrane, physiology and biochemistry. Clinical Haematology 1972; 1: 235
• 18 Huang T. W, Lagunoff D, Benditt E. P. Nonaggregative adherence of platelets to basal lamina in vitro. Laboratory Investigation 1974; 31: 156
• 19 Hugues J, Mahieu P. Platelet aggregation induced by basement membranes. Thrombosis et Diathesis Haemorrhagica 1970; 24: 395
• 20 Hunter W. M, Greenwood F. C. Preparation of iodine-131 labelled human growth hormone of high specific radioactivity. Nature (London) 1962; 194: 495
• 21 Jaffe R, Deykin D. Evidence for a structural requirement for the aggregation of platelets by collagen. Journal of Clinical Investigation 1974; 53: 875
• 22 Jaffe R, Deykin D. Binding of collagen to platelets. Thrombosis et Diathesis Haemorrhagica (Abstracts) 1975; 34: 332
• 23 Jamieson G. A, Smith D. F, Kosaw D. P. Possible role of collagen: Glucosyltransferase in platelet adhesion. Mechanistic considerations. Thrombosis et Diathesis Haemorrhagica 1975; 33: 668
• 24 Jamieson G. A, Urban C. L, Barber A. J. Enzymatic basis for platelet: collagen adhesion as the primary step in haemostasis. Nature, New Biology 1971; 234: 5
• 25 Kronick P. L, Jimenez S. A. Quantitative determination of collagen-platelet binding. Thrombosis et Diathesis Haemorrhagica (Abstracts) 1975; 34: 332
• 26 Macintyre D. E, Gordon J. L. Factors influencing the binding of radiolabelled collagen to blood platelets. Thrombosis et Diathesis Haemorrhagica (Abstracts) 1975; 34: 332
• 27 Muggli R, Baumgartner H. R. Collagen induced platelet aggregation: requirement for tropocollagen multimers. Thrombosis Research 1973; 3: 715-728.
• 28 Mustard J. F, Packham M. A. Factors influencing platelet function: adhesion release and aggregation. Pharmacological Reviews 1970; 22: 97
• 29 Nossel H. L, Wilner G. D, Leroy E. C. Importance of polar groups for initiating blood coagulation and aggregating platelets. Nature (London) 1969; 221: 75
• 30 Puett D, Wassermann B. K, Ford J. D, Cunningham L. W. Collagen mediated platelet aggregation: effects of collagen modification involving the protein and carbohydrate moieties. Journal of Clinical Investigation 1973; 52: 2495
• 31 Roseman S. The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion. Chemistry & Physics of Lipids 1970; 5: 270
• 32 Schwartz A, Stenzel K. H, Kohno I, Ast D, Minyata T, Rubin A. L. Deposition of blood cells on collagen and cuprophane membranes. Journal of Biomedical Materials Research 1975; 9: 453
• 33 Spaet T. H, Lejnieks I. A technique for estimation of platelet-collagen adhesion. Proceedings of the Society of Experimental Biology & Medicine 1969; 132: 1038
• 34 Wang C. L, Miyata T, Schlear S, Weksler B, Rubin A. L, Stenzel K. H. Collagen and glomerular basement membrane effects on platelets. Transactions of the American Society for Artificial Internal Organs 1975; 21: 422
• 35 Weiss H. J. Platelet physiology and abnormalities of platelet function. New England Journal of Medicine 1975; 293: 531
• 36 Wllner G. D, Nossel H.L, Procupez T. L. Aggregation of platelets by collagen: polar active sites of insoluble human collagen. American Journal of Physiology 1971; 220: 1074
• 37 Wilner G. D, Nossel H. L, Leroy E. C. Aggregation of platelets by collagen. Journal of Clinical Investigation 1968; 47: 2616