Potentiation by α and Inhibition by β-Adrenergic Stimulations of Rat Platelet Aggregation

S. K Yu; J. G Latour

doi:10.1055/s-0038-1649249

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 37(03): 413-422
DOI: 10.1055/s-0038-1649249

Original Article

Schattauer GmbH

Potentiation by α and Inhibition by β-Adrenergic Stimulations of Rat Platelet Aggregation

A Comparative Study with Human and Rabbit Platelets

Authors

S. K Yu^*

¹Laboratory of Experimental Pathology, Institut de Cardiologie de Montréal, and Department of Pathology, Université de Montréal, Montreal, Quebec, Canada
J. G Latour^**

¹Laboratory of Experimental Pathology, Institut de Cardiologie de Montréal, and Department of Pathology, Université de Montréal, Montreal, Quebec, Canada

Abstract

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Summary

Epinephrine, known to potentiate and elicit aggregation of human platelets, was shown to inhibit thrombin-induced aggregation of rat platelets, delaying the onset of aggregation from 2 to 12 times. Incubation of rat platelet suspensions with propranolol (1.25–30 μM), inactive by itself, totally prevented the inhibitory effect of epinephrine and also permitted a potentiation effect to show up. On the contrary, phentolamine (1.25–30 μM) potentiated the inhibitory effect of epinephrine on rat platelets and unmasked an inhibitory effect on human platelets. Finally, isoproterenol (0.25–9 μM) produced a marked inhibition of aggregation induced by thrombin, ADP and collagen in the three species studied, but most particularly in the rat. From these results, we conclude that stimulation of the platelet adrenergic receptors may either result in promotion (α-stimulation) or inhibition (β-stimulation) of platelet aggregation. Furthermore, differences in the ratios or responses of α/β receptors may account for species variations in the platelet aggregation response to catecholamine challenge.

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References

References
1 Abdulla Y. H. Beta-adrenergic receptors in human platelets. Journal of Atherosclerosis Research 1969; 9: 171
2 Ardlie N. G, Packham M. A, Mustard J. F. Adenosine diphosphate-induced platelet aggregation in suspensions of washed rabbit platelets. British Journal of Haematology 1970; 19: 7
3 Ardlie N. G, Glew G, Schwartz C. J. Influence of catecholamines on nucleotide-induced platelet aggregation. Nature 1966; 212: 415
4 Aster R. M, Jandl J. H. Platelet sequestration in man. I. Method. Journal of Clinical Investigation 1964; 43: 843
5 Ball G, Brereton G. G, Fulwood M, Ireland D. M, Yates P. Effect of prostaglandin Ei alone and in combination with theophylline or aspirin on collagen-induced platelet aggregation and on platelet nucleotides including adenosine 3’,5’ AMP. Biochemical Journal 1970; 120: 709
6 Brodie G. N, Baenziger N. L, Chase L. R, Majerus P. W. The effects of thrombin on adenyl cyclase activity and a membrane protein from human platelets. Journal of Clinical Investigation 1972; 51: 81
7 Bygdeman S, Johnsen O. Studies on the effect of adrenergic blocking drugs on catecholamine-induced platelet aggregation and uptake of noradrenaline and 5-hydroxytryptamine. Acta Physiologica Scandinavica 1969; 75: 129
8 Clayton S, Cross M. J. The aggregation of blood platelets by catecholamines and by thrombin. Journal of Physiology 1963; 169: 82
9 Gibelli A, Montanari C, Bellani D, Mandelli V, Sacchetti G. Beta-blocking drugs and human platelet aggregation in vitro. Experientia 1973; 29: 186
10 Haslam R. J. Roles of cyclic nucleotides in platelet function. In: Biochemistry and Pharmacology of platelets. Ciba Foundation Symposium 35, Elsevier, Excerpta Medica, North Holland Co. 1975: 121
11 Haslam R.J, Taylor A. Effects of catecholamines on the formation of adenosine 3’,5’cyclic AMP in human blood platelets. Biochemical Journal 1971; 125: 377
12 Latour J. G, Leger C. Prevention by glucocorticoids of disseminated intravascular coagulation induced by endotoxin: Mechanisms. The Journal of Laboratory and Clinical Medicine 1975; 85: 934
13 MacMillan D. C. Secondary clumping effect in human citrated platelet-rich plasma produced by adenosine diphosphate and adrenaline. Nature 1966; 211: 140
14 MacMillan D. G, Sim A. K. A comparative study of platelet aggregation in man and laboratory animals. Thrombosis et Diathesis Haemorrhagica 1970; 24: 385
15 Mannucci P. M, Pareti F. I. Platelet functions after intravenous administration in man of cyclic AMP and related drugs. Journal of Laboratory and Clinical Medicine 1974; 84: 828
16 Marquis N. R, Becker J. A, Vigdahl R. L. Platelet aggregation. III. An epinephrine induced decrease in cyclic AMP synthesis. Biochemical and Biophysical Research Communications 1970; 39: 783
17 Marquis N. R, Vigdahl R. L, Tavormina P. A. Platelet aggregation. I. Regulation by cyclic AMP and prostaglandin E₁ . Biochemical and Biophysical Research Communications 1969; 36: 965
18 Mills D. C. B. Factors influencing the adenyl cyclase system in human blood platelets. In: S. Sherry and A. Scriabine (Eds.). Platelets and Thrombosis. University Park Press, p. 1974: 45
19 Mills D. C. B, Smith J. B. The influence of platelet aggregation of drugs that affect the accumulation of adenosine 3’,5’-cyclic monophosphate in platelets. Biochemical Journal 1971; 121: 185
20 Mills D. C. B, Robb I. A, Roberts G. C. K. The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation. Journal of Physiology 1968; 195: 715
21 Mills D. C. B, Roberts G. C. K. Effects of adrenaline on human blood platelets. Journal of Physiology 1967; 193: 443
22 Mitchell J.R. A, Sharp A. A. Platelet clumping in vitro. British Journal of Haematology 1964; 10: 78
23 Moskowitz J, Harwood J. P, Reid W. D, Krishna G. The interaction of norepinephrine and PGE₁ on the adenyl cyclase system of human and rabbit platelets. Biochimica et Biophysica Acta 1971; 230: 279
24 Mustard J. F, Perry D. W, Ardlie N. G, Packham M. A. Preparation of suspensions of washed platelets from humans. British Journal of Haematology 1972; 22: 193
25 Mustard J. F, Packham M. A. Factors influencing platelet function: Adhesion,release, and aggregation. Pharmacological Reviews 1970; 22: 97
26 O’Brien J. R. Some effects of adrenaline and antiadrenaline compounds on platelets in vitro and in vivo. Nature 1963; 200: 763
27 Robison G. A, Arnold A, Hartman R. C. Divergent effects of epinephrine and PGE₁ on the level of cyclic AMP on human platelets. Pharmacological Research Communications 1969; 1: 235
28 Sacchetti G, Bellani D, Montanari G, Gibelli A. Effects “in vitro” of some cardiovascular drugs and other agents on human platelet aggregation. Thrombosis et Diathesis Haemorrhagica 1973; 29: 190
29 Salzman E. W. Cyclic AMP and platelet function. New England Journal of Medicine 1972; 286: 358
30 Salzman E. W, Neri L. L. Cyclic3’, 5’-adenosine monophosphate in human blood platelets. Nature 1969; 224: 609
31 Sharma H. M, Panganamala R. V, Geer J. C, Cornwell D. G. Inhibition of platelet aggregation induced by either epinephrine or arachidonic acid. Thrombosis Research 1975; 7: 879
32 Sinakos Z, Caen J. Platelet aggregation in mammalians (Human,rat, rabbit, Guinea-pig, horse, dog). A comparative study. Thrombosis et Diathesis Haemorrhagica 1967; 17: 99
33 Sinakos Z, Caen J. Le comportement des plaquettes chez le rat. Thrombosis et Diathesis Haemorrhagica 1966; 16: 1963
34 Stock K, Westermann E. Competitive and non-competitive inhibition of lipolysis by a and ß-adrenergic blocking agents, methoxamine derivatives and prostaglandin E₁ . Life Sciences 1966; 5: 1667
35 Thomas D.P, Niewiarowski S, Ream V. Release of adenine nucleotides and platelet factor 4 from platelets of man and other species. Journal of Laboratory and Clinical Medicine 1970; 75: 608
36 Thomas D. P. The role of platelet catecholamines in the aggregation of platelets by collagen and thrombin. In: Platelets and Hemostasis. Experimental Biology and Medicine 1968; 3: 129
37 Zieve P. D, Greenough W. B. Adenyl cyclase in human platelets: Activity and responsiveness. Biochemical and Biophysical Research Communications 1969; 35: 462