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Thromb Haemost 1972; 27(01): 121-133
DOI: 10.1055/s-0038-1649347
Originalarbeiten — Original Articles — Travaux Originaux
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On the Mechanism by which Cell Contact Induces the Release Reaction of Blood Platelets; the Effect of Cationic Polymers

P Massini
1   Theodor Kocher Institute, University of Berne
,
E. F Lüscher
1   Theodor Kocher Institute, University of Berne
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Publication History

Publication Date:
29 June 2018 (online)

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Summary

Human blood platelets are aggregated by the basic polymers polylysine and DEAE- dextran. Under certain conditions a second phase of aggregation, concomitant with the release reaction, is elicited. The presence of ADP, calcium ions and a plasmatic cofactor within the primary aggregates are necessary for the induction of the release reaction. These experiments demonstrate that cell contact per se does not lead to a release reaction ; in order to become effective it must take place in the presence of ADP.

 

  • References

  • 1 Ardlie N. G, Perry D. W, Packham M. A, Mustard J. F. Influence of apyrase on stability of suspensions of washed rabbit platelets. Proc. Soc. exp. Biol. (N.Y) 136: 1021 1971;
    CrossrefPubMedGoogle Scholar
  • 2 Bettex-Galland M, Lüscher E. F. Studies on the metabolism of human blood platelets in relation to clot retraction. Thrombos. Diathes. haemorrh. (Stuttg) 04: 178 1960;
    PubMedGoogle Scholar
  • 3 Booyse F. M, Rafelson M. E. Human platelet contractile proteins: location, properties and function. Ser. Haematol 04: 152 1971;
    PubMedGoogle Scholar
  • 4 Born G. V. R. Effects of ADP and related substances on the adhesiveness of platelets in vitro and in vivo. Brit. J. Haemat 12: 37 1966;
    CrossrefPubMedGoogle Scholar
  • 5 Born G. V. R. Observations on the change in shape of blood platelets brought about by adenosine diphosphate. J. Physiol. (Lond) 209: 487 1970;
    CrossrefPubMedGoogle Scholar
  • 6 Born G. V. R, Gross M. J. The aggregation of blood platelets. J. Physiol. (Lond) 168: 178 1963;
    CrossrefPubMedGoogle Scholar
  • 7 Boyer P. D. The inhibition of pyruvate kinase by ATP: a Mg2+-buffer system for use in enzyme studies. Biochem. biophys. Res. Commun 34: 702 1969;
    CrossrefPubMedGoogle Scholar
  • 8 Da Prada M, Pletscher A. Synthesis and storage of nucleotides in blood platelets. Life Sei. pt. II 09: 1271 1970;
    PubMedGoogle Scholar
  • 9 Davey M. G, Lüscher E. F. Release reactions of human platelets induced by thrombin and other agents. Biochim. biophys. Acta (Amst) 165: 490 1968;
    CrossrefPubMedGoogle Scholar
  • 10 Gröttum K. A. Platelet surface charge and aggregation. Effects of polyelectrolytes. Thrombos. Diathes. haemorrh. (Stuttg) 21: 450 1969;
    PubMedGoogle Scholar
  • 11 Haslam R. J. Role of adenosine diphosphate in the aggregation of human blood platelets by thrombin and by fatty acids. Nature (Lond) 202: 765 1964;
    CrossrefPubMedGoogle Scholar
  • 12 Holmsen H. Adenine nucleotide metabolism in platelets and plasma. In: Biochemistry of blood platelets. ed. Kowalski E, Niewiarowski S. Acad. Press; London: 1967: 81.
    Google Scholar
  • 13 Holmsen H, Day H. J, Storm E. Adenine nucleotide metabolism of blood platelets VI : Subcellular localization of nucleotide pools with different functions in the platelet release reaction. Biochim. biophys. Acta (Amst) 186: 254 1969;
    CrossrefPubMedGoogle Scholar
  • 14 Holmsen H, Day H. J, Stormorken H. The blood platelet release reaction. Scand. J. Haematol. Suppl 08: 1 1969;
    PubMedGoogle Scholar
  • 15 Holmsen H, Rozenberg M. C. Adenine nucleotide metabolism of blood platelets III : adenine phosphoribosyl transferase and nucleotide formation from exogenous adenine. Biochim. bio- phys. Acta (Amst.) 157 : 266 1968;
    PubMedGoogle Scholar
  • 16 Ireland D. M. Effect of thrombin on the radioactive nucleotides of human washed platelets. Biochem. J 105: 857 1967;
    CrossrefPubMedGoogle Scholar
  • 17 Jenkins C. S. P, Packham M. A, Kinlough-Rathbone R, Mustard J. F. Interactions of polylysine with platelets. Blood 37: 395 1971;
    PubMedGoogle Scholar
  • 18 Jerushalmy Z, Zucker M. B. Some effects of fibrinogen degradation products (FDP) on blood platelets. Thrombos. Diathes. haemorrh. (Stuttg) 15: 413 1966;
    PubMedGoogle Scholar
  • 19 Keller R, Mueller-Eckhardt G, Kayser F. H, Keller H. U. Interrelations between different types of cells. I. A comparative study of the biological properties of a cationic polypeptide from lysosomes of polymorphonuclear leukocytes and other cationic compounds. Int. Arch. Allergy 33: 239 1968;
    CrossrefPubMedGoogle Scholar
  • 20 Macmillan D. C. Secondary clumping effect in human citrated platelet-rich plasma produced by ADP and adrenaline. Nature (Lond) 211: 140 1966;
    CrossrefPubMedGoogle Scholar
  • 21 Macmillan D. C, Oliver M. R. The initial changes in platelet morphology following the addition of adenosine diphosphate. J. Atheroscler. Res 05: 440 1965;
    CrossrefPubMedGoogle Scholar
  • 22 Markwardt F, Hoffmann A. Influence of fermentative changes of adenine nucleotides on the aggregation of platelets in blood plasma. Naturwiss 51: 388 1964;
    CrossrefPubMedGoogle Scholar
  • 23 Marshall E. F, Stirling G. S, Tait A. G, Todrick A. The effect of iproniazid and imipramine on the blood platelet 5-hydroxytryptamine level in man. Brit. J. Pharmacol 15: 35 1960;
    PubMedGoogle Scholar
  • 24 Massini P. Specific activity of 14C-labelled serotonin from human blood platelets. Experientia (Basel) 20: 87 1970;
    PubMedGoogle Scholar
  • 25 Massini P, Lüscher E. F. The induction of the release reaction in human blood platelets by close cell contact. Thrombos. Diathes. haemorrh. (Stuttg) 25: 13 1971;
    PubMedGoogle Scholar
  • 26 Mueller-Eckhardt G. h, Lüscher E. F. Immune reactions of human blood platelets, I. A comparative study on the effects on platelets of heterologous antiplatelet antiserum, antigen- antibody complexes, aggregated gammaglobulin and thrombin. Thrombos. Diathes. haemorrh. (Stuttg) 20: 155 1968;
    PubMedGoogle Scholar
  • 27 Mustard J. F, Perry D. W, Ardlie N. G, Packham M. A. Preparations of suspensions of washed platelets from humans. Personal communication. 1971
    PubMedGoogle Scholar
  • 28 O’Brien J. R. Platelet aggregation. II. Some results from a new method of study. J. clin. Pathol 15: 452 1962;
    CrossrefPubMedGoogle Scholar
  • 29 O’Brien J. R. Changes in platelet membranes possibly associated with platelet stickiness. Nature (Lond) 212: 1057 1966;
    CrossrefPubMedGoogle Scholar
  • 30 O’Brien J. R. Platelet function: a guide to platelet membrane structure. Ser. Haematol 03: 68 1970;
    PubMedGoogle Scholar
  • 31 O’Brien J. R, Woodhouse M. A. Platelets : their size, shape and stickiness in vitro : degranulation and propinquity. Exp. Biol. Med. (Basel) 03: 90 1968;
    PubMedGoogle Scholar
  • 32 Pfleiderer T. h, Brossmer R. Zum Mechanismus der Thrombozytenaggregation. II. Erhöhung der Klebrigkeit durch geladene Makromoleküle. Thrombos. Diathes. haemorrh. (Stuttg) 18: 674 1967;
    PubMedGoogle Scholar
  • 33 Schneider W, Kühler W, Gross R. Induction of blood platelet aggregation by cationic polypeptides. Thrombos. Diathes. haemorrh. (Stuttg) 19: 207 1968;
    PubMedGoogle Scholar
  • 34 Spaet T. H, Cintron J, Spivack M. Some properties of the platelet-connective tissue mixed agglutination reaction. Proc. Soc. exp. Biol. (N.Y) Ill: 292 1962;
    PubMedGoogle Scholar
  • 35 Stacey R. S. Uptake of 5-hydroxytryptamine by platelets. Brit. J. Pharmacol 16: 284 1961;
    PubMedGoogle Scholar
  • 36 Thomas D. P, Niewiarowski S, Ream V. J. Release of adenine nucleotides and platelet factor 4 from platelets of man and four other species. J. Lab. clin. Med 75: 607 1970;
    PubMedGoogle Scholar
  • 37 Weiss H. J, Aledort L. M, Kochwa S. The effect of salicylates on the hemostatic properties of platelets in man. J. clin. Invest 47: 2169 1968;
    CrossrefPubMedGoogle Scholar
  • 38 Zucker M. B, Borelli J. Reversible alterations in platelet morphology produced by anticoagulants and by cold. Blood 09: 602 1954;
    PubMedGoogle Scholar
  • 39 Zucker M. B, Peterson J. Serotonin, platelet factor 3-activity and platelet aggregating agent released by ADP. Blood SO: 556 1967;
    PubMedGoogle Scholar
  • 40 Zucker M. B, Peterson J. Inhibition of ADP-indnced secondary aggregation and other platelet functions by acetyl salicylic acid ingestion. Proc. Soc. exp. Biol. (N.Y) 127: 547 1968;
    CrossrefPubMedGoogle Scholar