On Intraocular Fibrinolysis

M Pandolfi; Inga Marie Nilsson; J. E Niléhn

doi:10.1055/s-0038-1649419

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1966; 15(01/02): 161-172
DOI: 10.1055/s-0038-1649419

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

On Intraocular Fibrinolysis^[*]

Authors

M Pandolfi

¹Coagulation Laboratory, Department of Medicine, University of Lund, Allmänna Sjukhuset, Malmö, Sweden
Inga Marie Nilsson

¹Coagulation Laboratory, Department of Medicine, University of Lund, Allmänna Sjukhuset, Malmö, Sweden
J. E Niléhn

¹Coagulation Laboratory, Department of Medicine, University of Lund, Allmänna Sjukhuset, Malmö, Sweden

Abstract

Summary

1. Intraocular fibrinolysis can be induced in rabbits by intravenous administration of streptokinase and plasminogen. Enhancement of intraocular fibrinolysis, however, requires a substantial increase in the fibrinolytic activity of the blood. Hence any attempt to induce therapeutic intraocular fibrinolysis by injection of fibrinolytic enzymes without checking the degree of fibrinolysis in the blood is meaningless.

2. The blood-aqueous barrier did not show any significant change during fibrinolytic activation.

3. Fibrinolytic split products could not be demonstrated in primary and plasmoid aqueous humour, but they were found in plasmoid aqueous after treatment of the animals with streptokinase and plasminogen.

4. Intraocular fibrinolysis can be inhibited in primary and plasmoid aqueous by parenteral administration of ε-ACA.

Full Text

References

References
1 Anjou G. I. N, Krakau C. E. T. A photographic method for measuring the aqueous flare of the eye in normal and pathological conditions. Acta ophtal. (Kbh) 38: 178 1960;
2 Berger B. The effect of streptokinase irrigation on experimentally clotted blood in the anterior chamber of human eyes. Acta ophthal. (Kbh) 40: 373 1962;
3 Bielawiec M, Nilsson I. M. Fibrinolysis and the plasma protein electrophoretic pattern. Scand. J. clin. Lab. Invest 16: 513 1964;
4 Binder R. F, Binder H. F, Gerras T, Wahl S. Clotting pattern in the aqueous humor. Arch. Ophthal 69: 389 1963;
5 Blatt W. F, Segal H, Gray J. L. Purification of streptokinase and human plasmin and their interaction. Thrombos. Diathes. haemorrh. (Stuttg) 11: 393 1964;
6 Chandler M. R, Rosenthal E. The effect of intramuscularly administered trypsin on blood injected into the vitreous of rabbits. Arch. Ophthal 59: 706 1958;
7 Daily L, Tuttle W. S. Experimental use of enzymes for hyphemas and conjunctival hematomas. Arch. Ophthal 65: 410 1961;
8 Fearnley G. R, Balmforth G, Fearnley E. Evidence of a diurnal fibrinolytic rhythm; with a simple method of measuring natural fibrinolysis. Clin. Sci 16: 645 1957;
9 Fletcher A. P, Sherry S, Alkjaersig N, Smyrniotis F. E, Jick S. The maintenance of a sustained thrombolytic state in man. II. Clinical observations on patients with myocardial infarction and other thrombo-embolic disorders. J. clin. Invest 39: 1111 1959;
10 Friedman M. W. Streptokinase in ophthalmology. Amer. J. Ophthal 35: 1184 1952;
11 Hopen J. M, Gampagna F. N. Use of intramuscular trypsin in traumatic, inflammatory and hemorrhagic ocular disturbances. Amer. J. Ophthal 40: 209 1955;
12 Hörven I. The effect of fibrinolysin (plasmin) on blood injected into the anterior chamber of the eye in rabbits. Acta Ophthal 39: 44 1961;
13 Hörven I. Fibrinolysis and hyphema. The effect of »Thrombolysin« and »Kabikinase« on clotted blood in camera anterior of the eye in rabbits. Acta Ophthal 40: 320 1962;
14 Hörven I, Opsal R. Fibrinolysis and hyphema. Acta Ophthal 42: 957 1964;
15 Hurwitz P. Buccal Varidase in ophthalmology. Amer. J. Ophthal 48: 823 1959;
16 Jaeger W. Experimentelle Untersuchungen über fibrinolyse im Kammarwasser. Ber. dtsch. ophthal. Ges 59: 163 1955;
17 Jukofsky S. L. A new technique in the treatment of hyphema. Amer. J. Ophthal 34: 1692 1951;
18 Keeney A. H, Zaki H. A. The role of trypsin in experimentally induced hyphema. Amer. J. Ophthal 43: 275 1957;
19 Kline D. L. The purification and crystallization of plasminogen (profibrinolysin). J. Biol. Chem 204: 949 1953;
20 Liebmann S. D, Pollen A, Podos S. M. Treatment of experimental total hyphema with intraocular fibrinolytic agents. Arch. Ophthal 68: 72 1962;
21 Liegl O. Trypsinbehandlung in der Ophthalmologie. Klin. Mbl. Augenheilk 132: 486 1958;
22 Morton R. W, Turnbull W. The effect of intracameral fibrinolysin on the rabbit cornea. Amer. J. Ophthal 57: 280 1964;
23 Niléhn J.-E, Nilsson I. M. Demonstration of fibrinolytic split products in human serum by an immunological method in spontaneous and induced fibrinolytic states. Scand. J. Haemat 01: 313 1964;
24 Nilsson I. M, Björkman S. E, Andersson L. Clinical experiences with å-aminocaproic acid (å-ACA) as an antifibrinolytic agent. Acta med. scand 170: 487 1961;
25 Nilsson I. M, Olow B. Fibrinolysis induced by streptokinase in man. Acta chir. scand 123: 247 1962;
26 Oncley J. L, Melin M, Richert D. A, Cameron J. W, Gross Jr. P. M. The separation of the antibodies, isoagglutinins, prothrombin, plasminogen and lipoprotein into subfractions of human plasma. J. Amer. chem. Soc 71: 541 1949;
27 O’Rourke J. F. An evaluation of intraocular streptokinase Amer. J. Ophthal 39: 119 1955;
28 Pandolfi M, Nilsson I. M, Martinsson G. Coagulation and fibrinolytic components in primary and plasmoid aqueous humour of rabbit. Acta ophthal. (Kbh) 42: 820 1964;
29 Paraskevas M, Nilsson I. M, Martinsson G. A method for determining serum inhibitors of plasminogen activation. Scand. J. clin. Lab. Invest 14: 138 1962;
30 Pierse D, Le Griece H. Urokinase in Ophthalmology. Lancet II: 1143 1963;
31 Podos S, Liebman S, Pollen A. Treatment of experimental total hyphemas with intraocular fibrinolytic agents. II. Arch. Ophthal 71: 537 1964;
32 Sacks-Wilner A, Sinclaire S, Boyes T. Streptokinase and streptodornase in intraocular hemorrhage. Amer. J. Ophthal 39: 730 1955;
33 Scheie H. G, Ashley B. J, Weiner A. The treatment of total hyphema with fibrinolysin (plasmin). Arch. Ophthal 66: 226 1961;
34 Scheie H. G, Ashley B. J, Burns D. T. Treatment of total hyphema with fibrinolysin. Arch. Ophthal 69: 147 1963;
35 Schenk H, Trichtet F. Experimentelle Untersuchungen zur Frage der Resorption von Blut in der Vorderkammer durch Trypsin. Ophthalmologica 134: 396 1957;
36 Schimek R. A, Steffensen E. H. Vitreous hemorrhage absorption. Amer. J. Ophthal 39: 677 1955;
37 Sherry S. Fibrinolysis and clinical medicine. Triangle 06: 294 1964;
38 Shulman S, Alkjaersig N, Sherry S. Physicochemical studies on human plasminogen (profibrinolysin) and plasmin (fibrinolysin). J. Biol. Chem 233: 91 1958;
39 Sinskey R. M, Krichesky A. R. Experimental hyphema in rabbits. IV. The effect of fibrinolytic enzymes on the rate of absorption. Amer. J. Ophthal 54: 445 1962;
40 Smillie J. W. The effect of streptokinase on simulated hyphema. Amer. J. Ophthal 37: 911 1954;

On Intraocular Fibrinolysis[*]

Authors

On Intraocular Fibrinolysis^[*]