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Thromb Haemost 1995; 74(03): 859-863
DOI: 10.1055/s-0038-1649837
Original Article
Clinical Studies
Schattauer GmbH Stuttgart

Circulating Dermatan Sulfate and Heparan Sulfate/Heparin Proteoglycans in Children Undergoing Liver Transplantation

Lesley Mitchell
1   The Department of Pediatrics, Children’s Hospital at Chedoke-McMaster, Hamilton, Ontario, Canada
,
Ricardo Superina
2   The Department of Surgery, The Hospital for Sick Children, Toronto, Ontario, Canada
,
Michael Delorme
3   The Department of Medicine, St. Joseph’s Health Centre, London, Ontario, Canada
,
Patsy Vegh
1   The Department of Pediatrics, Children’s Hospital at Chedoke-McMaster, Hamilton, Ontario, Canada
,
Leslle Berry
1   The Department of Pediatrics, Children’s Hospital at Chedoke-McMaster, Hamilton, Ontario, Canada
,
Hugh Hoogendoorn
4   The Department of Medicine, McMaster University Medical Centre, Hamilton, Ontario, Canada
,
Maureen Andrew
1   The Department of Pediatrics, Children’s Hospital at Chedoke-McMaster, Hamilton, Ontario, Canada
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 06. Februar 1995

Accepted after resubmission 18. Mai 1995

Publikationsdatum:
09. Juli 2018 (online)

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Summary

The liver produces dermatan sulfate (DS), heparan sulfate (HS) and heparin glycosaminoglycans (GAG) and in the presence of hepatic disease, tissue levels of the DS GAG increase dramatically. We hypothesized that in children undergoing liver transplantation plasma levels of DS would be increased. Plasma from children undergoing liver transplantation were tested preoperative, intra operative and post operative at 24-48 h, and 1-3 weeks. Fluctuating levels of DS, HS and heparin anticoagulant activity were detected at all timepoints. The anticoagulant activity was purified and gel chromatography of the material displayed a mean Mr 110,000 D. Reductive elimination decreased the mean Mr 24,000 D indicating the activity resides on a proteoglycan (PG). The purified material was subjected to further chromatography and two peaks of anticoagulant activity resolved, compatible with at least two separate PGs, one with DS GAG chains and the additional PG(s) with HS and heparin GAG chains.

 

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