Interaction of Echicetin with a High Affinity Thrombin Binding Site on Platelet Glycoprotein GPIb

Manling Peng; Frances A Emig; Ahua Mao; Weiqi Lu; Edward P Kirby; Stefan Niewiarowski; M Anna Kowalska

doi:10.1055/s-0038-1649854

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1995; 74(03): 954-957
DOI: 10.1055/s-0038-1649854

Original Article

Platelets

Schattauer GmbH Stuttgart

Interaction of Echicetin with a High Affinity Thrombin Binding Site on Platelet Glycoprotein GPIb

Authors

Manling Peng

¹The Sol Sherry Thrombosis Research Center, Philadelphia, PA, USA

²The Department of Biochemistry, Philadelphia, PA, USA
Frances A Emig

²The Department of Biochemistry, Philadelphia, PA, USA
Ahua Mao

³The Department of Pharmacology, Philadelphia, PA, USA
Weiqi Lu

¹The Sol Sherry Thrombosis Research Center, Philadelphia, PA, USA

⁴The Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA
Edward P Kirby

¹The Sol Sherry Thrombosis Research Center, Philadelphia, PA, USA

²The Department of Biochemistry, Philadelphia, PA, USA
Stefan Niewiarowski

¹The Sol Sherry Thrombosis Research Center, Philadelphia, PA, USA

⁴The Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA
M Anna Kowalska

¹The Sol Sherry Thrombosis Research Center, Philadelphia, PA, USA

⁴The Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA

Abstract

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Summary

Echicetin, a protein isolated from Echis carinatus snake venom, inhibited platelet aggregation and secretion induced by low concentrations of thrombin (<0.2 U/ml), by binding to platelet glycoprotein lb (GPIb). The inhibition was not observed when the platelets were stimulated with higher concentrations of thrombin (>0.2 U/ml). Echicetin competed with thrombin for binding to the high affinity site on GPIb. Thrombin also inhibited 50% of the binding of ¹²⁵I-echicetin to the platelets.

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Referenzen

References
1 Peng M, Lu W, Beviglia L, Niewiarowski S, Kirby EP. Echicctin: A snake venom protein dial inhibits binding of von Willebrand factor and albo- aggregins to platelet glycoprotein lb. hi nod 1993 81: 2321-2328
2 Peng M, Lu W, Kirby EP. Alboaggregin-B: A new platelet agonist that binds to platelet membrane glycoprotein lb. Biochemistry 1991; 30: l1529-36
3 Peng M, Lu W, Kirby EP. Characterization of three alboaggregins purified from Trimcresurus albolabris venom. Thromb Haemost 1992; 67: 702-707
4 Peng M, Holt JC, Niewiarowski S. Isolation, characterization and amino acid sequence ofechicetin β subunit, a specific inhibitor of von Willebrand factor and thrombin interaction with glycoprotein lb. Biochcm Biophys Res Comm 1994; 205: 68-72
5 Ganguly P. Binding of thrombin to human platelets. Nature 1974; 247: 306-307
6 Harmon JT, Jamieson GA. The glyeoealicin portion of platelet glycoprotein lb expresses both high and moderate affinity receptors for thrombin. A soluble radio-receptor assay for the interaction of thrombin with platelets J Biol Chem 1986; 261: 13224-13229
7 McGowan EB, Yeo KT, Detwiler TC. The action of calcium-dependent protease on platelet surface glycoproteins. Arch Biochcm Biophys 1983; 227: 287-301
8 Vu T-KH, Hung DT, Wheaton VI, Coughlin SR. Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanims of receptor activation. Cell 1991; 64: 1057-1068
9 Coughlin SR, Vu T-KH, Hung DT, Wheaton VI. Characterization of a functional thrombin receptor. J Clin Invest 1992; 89: 351-355
10 Harmon JT, Jamieson GA. Platelet activation by alpha-thrombin is a receptor-mediated event. Ann NY Acad Sci 1986; 485: 387-395
11 Martin BM, Wasiewski JW, Fenton IIJW, Detwiler TC. Equilibrium binding of thrombin to platelets. Biochemistry 1976; 15: 4886-4893
12 Berndt MC, Gregory C, Dowden G. Thrombin interaction with platelet membrane proteins. Ann NY Acad Sci 1986; 485: 374-386
13 De Marco L, Mazzucato M, Masotti A, Fenton II JW, Ruggeri ZM. Function of glycoprotein Ibα in platelet activation by α-thrombin. J Biol Chem 1991; 266: 23776-23783
14 Jamieson GA, Okumura T. Reduced thrombin binding and aggregation in Bernard-Soulier platelets. J Clin Invest 1978; 61: 861-864
15 Cooper HA, Bennett WP, White GC, 2nd Wagner GH. Hydrolysis of human platelet membrane glycoproteins with a Serratia marcescens metalloprotease: effect on response to thrombin and von Willebrand factor. Proc Natl Acad Sci USA 1982; 79: 1433-1437
16 McGowan EB, Detwiler TC. Differential effect of Serratia protease on platelet surface glycoproteins lb and V. Blood 1985; 65: 1033-1033
17 Mustard JF, Perry DW, Ardlie NG, Packham MA. Preparation of suspension of washed platelets from humans. Br J Haematol 1972; 22: 193-204
18 Rucinski B, Niewiarowski S, James P, Waltz DA, Budzynski AZ. Antiheparin proteins secreted by human plateletsPurification, characterization and radioimmunoassay. Blood 1979; 53: 47-62
19 De Marco L, Mazzucato M, Masotti A, Ruggeri ZM. Localization and characterization of an a-thrombin binding site on platelet glycoprotein Iba. J Biol Chem 1994; 269: 6478-6484
20 Handa M, Titani K, Holland LZ, Roberts JR, Ruggeri ZM. The von Willebrand factor-binding domain of platelet membrane glycoprotein lb: characterization by monoclonal antibodies and partial amino acid sequence analysis of proteolytic fragments. J Biol Chem 1986; 261: 12579-12585
21 Gralnick HR, Williams S, McKeown LP, Hansmann K, Fenton IIJW, Krutzsch H. High-affinity α-thrombin binding to platelet glycoprotein Ibα: Identification of two binding domains. Proc Natl Acad Sci USA 1994; 91: 6334-6338
22 Cruz M, Petersen E, Turci SM, Handin RI. Functional analysis of a glycoprotein Iba polypeptide which inhibits von Willebrand factor binding to the platelet glycoprotein Ib-IX complex and to collagen. J Biol Chem 1992; 267: 1303-1309
23 Brass LF, Vassallo Jr RR, Belmonte E, Ahuja M, Cichowski K, Hoxie JA. Structure and function of the human platelet thrombin receptor. J Biol Chem 1992; 267: 13759-13798