Some Immunofluorescent Observations on Factor VIII/von Willebrand Factor in Dogs

Elizabeth V Potter; Martha A Shaughnessy; David Green

doi:10.1055/s-0038-1650083

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1980; 44(02): 056-061
DOI: 10.1055/s-0038-1650083

Original Article

Schattauer GmbH Stuttgart

Some Immunofluorescent Observations on Factor VIII/von Willebrand Factor in Dogs

Authors

Elizabeth V Potter

The Sections of Nephrology-Hypertension and Hematology, Northwestern University Medical School and the Atherosclerosis Program, Rehabilitation Institute of Chicago, Chicago, Illinois, U.S.A.
Martha A Shaughnessy

The Sections of Nephrology-Hypertension and Hematology, Northwestern University Medical School and the Atherosclerosis Program, Rehabilitation Institute of Chicago, Chicago, Illinois, U.S.A.
David Green

The Sections of Nephrology-Hypertension and Hematology, Northwestern University Medical School and the Atherosclerosis Program, Rehabilitation Institute of Chicago, Chicago, Illinois, U.S.A.

Abstract

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Summary

Factor VIII/von Willebrand factor (vWF) was sought by immunofluorescence in or on canine platelets and blood vessels. None was found on normal canine platelets and little was present in normal canine arteries, veins and capillaries compared with normal human blood vessels. However, free granules of vWF were scattered in platelet-rich canine plasma and occasional granules appeared on small clumps of platelets when ristocetin or collagen was added to the plasma. When the same platelets were suspended in human plasma and ristocetin or collagen was added, more clumps were formed and more vWF (human) was associated with these clumps. When thrombin was added to canine platelets in either canine or human serum, more solid, small clumps of platelets were formed and stained with the anti-vWF sera. When thrombin was added to canine platelets in either canine or human plasma, a single large clot was formed which stained brightly for vWF.

Key words

Canine von Willebrand factor - Canine vWF - von Willebrand factor in dogs - vWF in dogs - Decreased platelet vWF in dogs

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References

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