Summary
Rat platelets were incubated in either balanced salt solution (BSS) buffered with
imidazole, in plasma or in a third medium composed of BSS and plasma in a volume to
volume mixture. Platelets incubated in imidazole BSS showed a more rapid decline in
platelet count, earlier appearance of platelet clumping, greater consumption of glucose,
greater production of lactic acid and CO2, and more rapid turnover of 32Pi. Platelets
incubated in plasma showed less pronounced changes in these parameters while platelets
incubated in imidazole BSS: plasma showed effects that were intermediate in degree
to those observed with imidazole BSS or plasma alone. The possibility that these effects
are related to the depletion of platelet ATP by imidazole is discussed.
