Fibrinogen Binding to ICAM-1 on EA.hy 926 Endothelial Cells Is Dependent on an Intact Cytoskeleton

Anja van de Stolpe; Nancy Jacobs; Willem J Hage; Leon Tertoolen; Yvette van Kooyk; Irena RO Nováková; Theo de Witte

doi:10.1055/s-0038-1650240

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1996; 75(01): 182-189
DOI: 10.1055/s-0038-1650240

Original Article

Schattauer GmbH Stuttgart

Fibrinogen Binding to ICAM-1 on EA.hy 926 Endothelial Cells Is Dependent on an Intact Cytoskeleton

Anja van de Stolpe

¹The Department of Hematology, University Hospital Nijmegen, The Netherlands

,

Nancy Jacobs

¹The Department of Hematology, University Hospital Nijmegen, The Netherlands

,

Willem J Hage

²The Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands

,

Leon Tertoolen

²The Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands

,

Yvette van Kooyk

³The Tumor Immunology Laboratory, University Hospital Nijmegen, The Netherlands

,

Irena RO Nováková

¹The Department of Hematology, University Hospital Nijmegen, The Netherlands

,

Theo de Witte

¹The Department of Hematology, University Hospital Nijmegen, The Netherlands

› Author Affiliations

Abstract

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Summary

Fibrinogen is a ligand for Intercellular Adhesion Molecule-1 (ICAM-1), and enhances monocyte-endothelial cell interaction by coupling Mac-1 on monocytes to ICAM-1 on endothelial cells. We investigated the role of the cytoskeleton in fibrinogen binding to the human endothelial cell line EA.hy 926 using immunofluorescence techniques. In this cell line TNFα induced the simultaneous appearance of stress fibers and of ICAM-1, which was clustered predominantly on endothelial cell projections. Incubation of TNFα-stimulated endothelial cells with fibrinogen resulted in binding of fibrinogen to ICAM-1 on these cell projections. Disruption of the cytoskeleton by cytocholasin B abolished fibrinogen binding. Activation of protein kinase C with 12-O-tetradecanoyl phorbol- 13-acetate resulted in simultaneous loss of both stress fibers and fibrinogen binding. These results suggest that a connection between ICAM-1 and the cytoskeleton results in clustering of ICAM-1 on cell projections, which is required for fibrinogen binding.

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References

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