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Thromb Haemost 1996; 75(04): 600-606
DOI: 10.1055/s-0038-1650329
Original Article
Schattauer GmbH Stuttgart

Increased Expression of Plasminogen Activator Inhibitor-1 in R.rickettssi-infected Endothelial Cells

Rui-Jin Shi
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York
,
Patricia J Simpson-Haidaris
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York
,
Victor J Marder
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York
,
David J Silverman
1   The Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, USA
,
Lee Ann Sporn
The Hematology Unit, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York
› Author Affiliations
Further Information

Publication History

Received 24 May 1995

Accepted after resubmission 04 January 1996

Publication Date:
10 July 2018 (online)

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Summary

Changes in PAI-1 expression in human umbilical vein endothelial cells (HUVEC) were studied following in vitro infection with Rickettsia rickettsii. A 1.8-fold increase in secreted PAI-1 activity occurred in infected versus control cultures (p = 0.03) at 24 h but not at earlier timepoints. A similar increase (1.4-fold) in secreted PAI-1 antigen (p <0.005) was measured by ELISA. To determine whether this increase was due to increased synthesis of PAI-1, HUVEC were metabolically labeled with 35S-methionine concurrent with R. rickettsii infection. Such infection resulted in a 1.9-fold increase in labeled PAI-1 in the medium at 24 h (p = 0.036). Increase in steady-state levels of PAI-1 mRNA were detected as early as 18 h by Northern blot analysis, peaking (5.5-fold) at approximately 24 h. These results indicate that PAI-1 production is increased in RR-infected endothelial cells, an effect that may contribute to the vascular occlusions noted in Rocky Mountain spotted fever.

 

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