Detection of Circulating Tissue Factor and Factor VII in a Normal Population

Sybille Albrecht; Matthias Kotzsch; Gabriele  Siegert; Thomas Luther; Heinz Großmann; Marianne Großer; Martin Müller

doi:10.1055/s-0038-1650365

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1996; 75(05): 772-777
DOI: 10.1055/s-0038-1650365

Original Article

Schattauer GmbH Stuttgart

Detection of Circulating Tissue Factor and Factor VII in a Normal Population

Sybille Albrecht

The Institut für Pathologie, Technische Universitat Dresden, Germany

,

Matthias Kotzsch

The Institut für Pathologie, Technische Universitat Dresden, Germany

,

Gabriele Siegert

¹Institut für Klinische Chemie und Laboratoriumsmedizin, Technische Universitat Dresden, Germany

,

Thomas Luther

The Institut für Pathologie, Technische Universitat Dresden, Germany

,

Heinz Großmann

The Institut für Pathologie, Technische Universitat Dresden, Germany

,

Marianne Großer

The Institut für Pathologie, Technische Universitat Dresden, Germany

,

Martin Müller

The Institut für Pathologie, Technische Universitat Dresden, Germany

› Author Affiliations

Abstract

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Summary

The plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation.

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References

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