Thromb Haemost 1996; 75(05): 782-790
DOI: 10.1055/s-0038-1650367
Original Article
Schattauer GmbH Stuttgart

Fibrinogen Promotes Adhesion of Monocytic to Human Mesothelioma Cells

Authors

  • Sreerama Shetty

    1   The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
  • Anuradha Kumar

    The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
  • Siegfried Pueblitz

    1   The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
  • Saleh Emri

    2   The Departments of Chest Diseases, Hacettepe University School of Medicine, Ankara, Turkey
  • Yucel Gungen

    3   Pathology, Hacettepe University School of Medicine, Ankara, Turkey
  • Alice R Johnson

    4   Biochemistry and Pathology, The University of Texas Health Center at Tyler, Tyler, TX, USA
  • Steven Idell

    1   The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
Further Information

Publication History

Received 06 September 1995

Accepted after resubmission 29 January 1996

Publication Date:
10 July 2018 (online)

Preview

Summary

Adhesion between monocytic and mesothelioma or pleural meso-thelial cells influences stromal remodeling in pleural neoplasia. We found that cultured monocytic cells (U937) adhere to either human pleural mesothelioma (MS-1) or mesothelial (MeT5A) cells in vitro. 125I-fibrinogen bound specifically and saturably to either cell line, and specific fibrinogen binding increased upon stimulation of these cells with proinflammatory agents such as phorbol myristate (PMA), lipo-polysaccharide (LPS) or tumor necrosis factor (TNF-±). We purified the fibrinogen receptor protein from a membrane fraction of MS-1 cells and identified it by immunoprecipitation as intercellular adhesion molecule (ICAM-1). Anti-ICAM-1 antibody or antisense oligonucleotides inhibited fibrinogen-mediated cell adhesion and binding of 125I-fibrinogen to mesothelioma or mesothelial cells. Cultured monocytic cells adhere to either mesothelioma or mesothelial cells, and the interaction is promoted by fibrinogen binding ICAM-1 at the cell surface. ICAM-1 is expressed by mesothelioma cells and CD 1 lb by macrophages in the fibrinous mesothelioma tumor stroma. The data suggest a common mechanism by which monocytic cells could adhere to either malignant mesothelioma cells or the mesothelial surface in pleural neoplasia.