Multicenter Comparison of Five Functional and Two Immunological Assays for Protein C

P M Mannucci; C Boyer; A Tripodi; S Viganò-D'Angelo; M Wolf; C Valsecchi; A D'Angelo; D Meyer; M J Larrieu

doi:10.1055/s-0038-1651059

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1987; 57(01): 044-048
DOI: 10.1055/s-0038-1651059

Original Articles

Schattauer GmbH Stuttgart

Multicenter Comparison of Five Functional and Two Immunological Assays for Protein C

Authors

P M Mannucci

¹The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
C Boyer

²The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France
A Tripodi

¹The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
S Viganò-D'Angelo

³The Thrombosis/Hematology Research Program Oklahoma Medical Research Foundation, Oklahoma City, USA
M Wolf

²The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France
C Valsecchi

¹The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
A D'Angelo

¹The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
D Meyer

²The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France
M J Larrieu

²The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France

Abstract

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Summary

Five functional assays and two immunoassays for protein C (PC) were evaluated in parallel for the same plasma samples collected from healthy subjects, patients with congenital and acquired PC deficiencies or patients with conditions associated with high PC levels. For 7 patients starting warfarin therapy and for 15 patients during stabilized warfarin therapy, there were significant between-assay differences. For these groups immunoassays gave higher values than most functional assays and the latter also gave varied results, probably depending on their respective capacity for recognizing acarboxylated PC. On the other hand, there were no significant between-assay differences nor discrepancies between PC activity and antigen levels for healthy subjects (n = 39), patients with congenital PC deficiency (n = 10), myocardial infarction (n = 25), chronic liver disease (n = 19), disseminated intravascular coagulation (n = 35), in the post-operative period (n = 20) or in women taking oral contraceptives (n = 20). This comparison of PC assays indicates that PC levels measured by different functional or immunological assays are very close in the majority of clinical conditions, but not for patients on oral anticoagulants.

Keywords

Protein C - Protein C assays

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References

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