Particulate Nature of Acid Phosphatase Released from Thrombocytes

Summary

Acid phosphatases are released from thrombocytes into serum during coagulation of native PRP or blood in particulate form. Latent activity probably masked in phospholipoprotein structures could be discovered with the use of detergent.

Some physicochemical properties are described with regard to the PF 3 like activity of serum.

Possible connection to platelet lysosomes is discussed.

• References

• 1 Marcus A.J, Zucker M.B. The physiology of blood platelets.. Grune and Stratton, Inc.; New York: 1965
• 2 Lüscher E.F. Platelets in haemostasis and thrombosis.. Brit. J. Haemat 13: 1
• 3 Zucker M.B, Borelli J. Platelets as a source of serum acid nitrophenylphosphatase.. J. clin. Invest 1959; 38: 148
• 4 Dohrmann R, Klesper R. Untersuchungen zur B-Glucuronidase-Aktivität menschlicher Thrombocyten.. Klin. Wschr 1960; 38: 595
• 5 Polášek J, Rotrekl B. Release of nitrocatecholsulphatase during blood coagulation from thrombocytes. Thrombos. Diathes. haemorrh. (Stuttg.).(To be published.).
• 6 Novik off A.B, Brachet J, Mirski A.E. Lysosomes and related particles in: The Cell. Vol. II. Edited by.. 1961. Academic Press; New York and London: 1967: 425
• 7 Marcus A.J, Zucker-Franklin D, Safier L.B, Ullman H.L. Studies on human platelet granules and membranes.. J. clin. Invest 1966; 45: 14
• 8 Jacobsson K. The determination of tartrate-inhibited phosphatase in serum.. Scand. J. clin. Lab. Invest 1960; 12: 367
• 9 Polášek J, Rotrekl B. Arylsulphatases of human blood platelets.. Nature (Lond.) 1967; 214: 187
• 10 O’Brien J.R. The platelet-like activity of serum.. Brit. J. Haemat 1955; I: 223
• 11 Alagille D, Soulier J.P. Le test d’activité plaquettaire du serum.. Rev. franç. Étud. clin. biol 1957; 2: 231
• 12 Rodman N.F, Mason R.G. Platelet-platelet interaction: relationship to hemostasis and thrombosis.. Fed. Proc 1967; 26: 95
• 13 Shinowara G.Y. Human thromboplastic cellular and tissue factors in blood coagulation: Lipoprotein release in vivo.. Acta haemat. jap 1961; 24: 716
• 14 Prince A.M, Adams W.R. Virus-like particles in human plasma and serum: Role of platelet lysosomes.. J. nat. Cancer Inst 1966; 37: 153
• 15 White J.G, Krivit W. The ultrastructural localization and release of platelet lipids.. Blood 1966; 27: 167
• 16 Wolf P. The nature and significance of platelet products in human plasma.. Brit. J. Haemat 1967; 13: 269
• 17 Iatridis P.G, Ferguson J.H. Effect of alkaline and acid phosphatases on clotting: Evidence that alkaline phosphatase enhances activation of factor IX by SF, and that acid phosphatase enhances plasmatic anti-SF.. Thrombos. Diathes. haemorrh. (Stuttg.) 1966; 15: 365
• 18 Sawant P.L, Shibko S, Kumta U.S, Tappel A.L. Isolation of rat-liver lysosomes and their general properties.. Biochim. biophys. Acta (Amst.) 1964; 85: 82