Hyperaggregability of Platelets in Thromboembolic Disorders

Hiroh Yamazaki; Takeo Takahashi; Tadahiro Sano

doi:10.1055/s-0038-1651449

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1975; 34(01): 094-105
DOI: 10.1055/s-0038-1651449

Original Article

Schattauer GmbH

Hyperaggregability of Platelets in Thromboembolic Disorders

Authors

Hiroh Yamazaki

¹3rd Department of Medicine, Tokyo Medical and Dental University, School of Medicine, Yushima, Tokyo, Japan
Takeo Takahashi

¹3rd Department of Medicine, Tokyo Medical and Dental University, School of Medicine, Yushima, Tokyo, Japan
Tadahiro Sano

¹3rd Department of Medicine, Tokyo Medical and Dental University, School of Medicine, Yushima, Tokyo, Japan

Abstract

Summary

Platelet aggregability was measured using platelet rich plasma (PRP) collected from 197 clinical cases including 52 healthy volunteers. In 31 patients of acute stage of thrombosis (within 2 weeks from the onset), a significant enhancement of platelet aggregation measured 5 min after an addition of 3 and 10 μM ADP or 0.1 and 1 μg/ml of adrenaline to PRP (p < 0.05, compared to the healthy group). Also a significant enhancement of secondary aggregation induced by adrenaline was observed (p <0.05). The enhancement was especially marked in the response induced by adrenaline. Such an enhancement was not observed in patients in the recovery stage of thrombosis, hypertension, angina pectoris and other miscellaneous diseases. There was no difference in the parameters related to the velocity of aggregation or intensity of primary aggregation between the diseased and the healthy group. In response induced by collagen (bovine achules tendon, 0.3 and 1 mg/ml) any difference in the aggregation curve was not observed between the diseased and the healthy group. Such findings suggest a presence of an enhancement of ADP-release mechanism of platelets in acute thrombosis. Also a significance of adrenaline-induced platelet aggregation was proposed to detect platelet functions for analysis of mechanism of thromboembolic disorders.

Full Text

References

References
1 Born G. V. R. 1962; Quantitative investigations into the aggregation of blood platelet. Journal of Physiology 162: 67.
2 Hardisty R. M, Hutton R. A, Montgomery D, Rickard S, and Trebilcock H. 1970; Secondary platelet aggregation: a quantitative study. British Journal of Haematology 19: 307.
3 Hovig T, Jorgensen L, Packham M. A, and Mustard J. F. 1968; Platelet adherence to fibrin and collagen. Journal of Laboratory and Clinical Medicine 71: 29.
4 Inceman S, and Tangün Y. 1972; Platelet defects in the myeloproliferative disorders. Annals of the New York Academy of Sciences 201: 251.
5 Macmillan D. C. 1966; Secondary clumping effect in human citrated platelet-rich plasma produced by adenosine diphosphate and adrenaline. Nature 211: 140.
6 Nomura T, and Onozawa Y. 1974. Platelet aggregation in myeloproliferative disorders. In: Didisheim P, Shimamoto T, and Yamazaki H. (eds.) Platelets, Thrombosis, and Inhibitors. F. K. Schattauer Verlag; Stuttgart: 247.
7 O’Brien J. R. 1962; Platelet aggregation - Part II. Some results from a new method of study. Journal of Clinical Pathology 15: 452.
8 O’Brien J. R, Heywood J. B, and Heady J. A. 1966; The quantitation of platelet aggregation induced by four compounds : a study in relation to myocardial infarction. Thrombosis et Diathesis Haemorrhagica 16: 752.
9 Rozenberg M. C, and Firkin B. G. 1966; The rate of platelet aggregation in haemor-rhagic disease and thrombosis. Scandinavian Journal of Haematology 3: 5.
10 Swank R. L. 1961; Alteration of blood on storage: measurement of adhesiveness of ‘aging’ platelets and leukocytes and their removal by filtration. New England Journal of Medicine 265: 728.
11 Yamazaki H, Kobayashi I, and Shimamoto T. 1970; a Enhancement of ADP-in-duced platelet aggregation by exercise test in coronary patients and its prevention by pyridinolcarbamate. Thrombosis et Diathesis Haemorrhagica 24: 438.
12 Yamazaki H, Sano T, Odakura T, Takeuchi K, and Shimamoto T. 1970; b Electrocardiographic and hematological changes by exercise test in coronary patients and pyridinolcarbamate pretreatment. A double-blind crossover trial. American Heart Journal 79: 640.
13 Yamazaki H, Numano F, Numano F, and Shimamoto T. 1972; /73 Estrogen and hyperaggregability of platelets measured by the screen filtration pressure (SFP) method in Takayasu’s disease. Haemostasis 1: 285.
14 Yamazaki H, Sano T, Shimamoto T, Mashimo N, Takahashi T, and Shimamoto T. 1974. Platelet functions in thromboembolic disorders. In: Didisheim P, Shimamoto T, and Yamazaki H. (eds.) Platelets, Thrombosis, and Inhibitors. F. K. Schattauer Verlag; Stuttgart: 213.
15 Zahavi J, and Dreyfuss F. 1969; An abnormal pattern of adenosine diphosphate-induced platelet aggregation in acute myocardial infarction. Thrombosis et Diathesis Haemorrhagica 21: 76.