PDF herunterladen
Thromb Haemost 1981; 46(03): 581-583
DOI: 10.1055/s-0038-1653422
Original Article
Schattauer GmbH Stuttgart

The In Vitro Inhibitory Effect on Thrombin by 2,3-Diphosphoglycerate

Authors

  • D Del Principe

    The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy

  • P M Strappini

    The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy

  • S Del Vecchio

    The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy

  • A Menichelli

    The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy

  • G Biancini

    The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy

  • E V Cosmi

    *   The Departments of Anesthesiology and Obstetrics and Gynecology, Medical School, University of Rome, Italy

  • V Bastianon

    The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy

  • C D’Arcangelo

    The Coagulation Laboratory, 1st Dept. of Pediatrics, Medical School, University of Rome, Italy
Weitere Informationen

Publikationsverlauf

Received 17. September 1980

Accepted 15. Juni 1981

Publikationsdatum:
05. Juli 2018 (online)

 als PDF herunterladen  Lizenzen und Reprints
Preview

Summary

Thrombin, incubated with 2,3-diphosphoglycerate (150 nmol 2,3-DPG/1 NIH thrombin unit) lost up to 70% of its clotting activity, whereas the esterase activity remained unchanged. No fibrinopeptide release by thrombin was observed in the presence of 2,3-DPG. The fibrin polymerization was normal. By chromatography on Amberlite IRC-50, α-thrombin was eluted at pH 8.0. In presence of 2,3-DPG, α-thrombin was not eluted. Likely, 2,3-DPG can interfere with thrombin.

Keywords

2,3-Diphosphoglycerate - Thrombin - Blood coagulation